Kang Yun-Joo, Shim Kyu-Chan, Lee Hyun-Sook, Jeon Yun-A, Kim Sun-Ha, Kang Ju-Won, Yun Yeo-Tae, Park In-Kyu, Ahn Sang-Nag
Department of Agronomy, College of Agriculture and Life Sciences, Chungnam National University, Daejeon, 34134, South Korea.
Department of Southern Area Crop Science, National Institute of Crop Science, Milyang, 50424, South Korea.
Genes Genomics. 2018 Apr;40(4):389-397. doi: 10.1007/s13258-017-0640-6. Epub 2017 Dec 19.
A quantitative trait locus (QTL) gw8.1 was detected in the population derived from a cross between the elite japonica cultivar, 'Hwaseong' and Oryza rufipogon (IRGC 105491). Near isogenic lines (NILs) harboring the O. rufipogon segment on chromosome 8 showed increased grain length and weight compared to those of the recurrent parent, Hwaseong. This QTL was mapped to a 175.3-kb region containing 28 genes, of which four were considered as candidates based on the presence of mutations in their coding regions and as per the RNA expression pattern during the inflorescence stage. Leaves and panicles obtained from plants harvested 5 days after heading showed differences in gene expression between Hwaseong and gw8.1-NILs. Most genes were upregulated in O. rufipogon and gw8.1-NIL than in Hwaseong. Scanning electron microscopy analysis of the lemma inner epidermal cells indicated that cell length was higher in gw8.1 NIL than in Hwaseong, indicating that gw8.1 might regulate cell elongation. Among the candidate genes, LOC_Os08g34380 encoding a putative receptor-like kinase and LOC_Os08g34550 encoding putative RING-H2 finger protein were considered as possible candidates based on their functional similarity.
在优良粳稻品种‘华城’与野生稻（IRGC 105491）杂交后代群体中检测到一个数量性状位点（QTL）gw8.1。在第8号染色体上携带野生稻片段的近等基因系（NILs）与轮回亲本‘华城’相比，粒长和粒重增加。该QTL被定位到一个175.3 kb的区域，包含28个基因，其中4个基因因其编码区存在突变以及根据花序期的RNA表达模式被视为候选基因。抽穗5天后收获的植株的叶片和穗在‘华城’和gw8.1-NILs之间表现出基因表达差异。大多数基因在野生稻和gw8.1-NILs中比在‘华城’中上调。对内外稃内表皮细胞的扫描电子显微镜分析表明，gw8.1 NIL中的细胞长度高于‘华城’，这表明gw8.1可能调控细胞伸长。在候选基因中，编码假定类受体激酶的LOC_Os08g34380和编码假定RING-H2指蛋白的LOC_Os08g34550基于其功能相似性被视为可能的候选基因。
