Beerelli Kavitha, Balakrishnan Divya, Addanki Krishnam Raju, Surapaneni Malathi, Rao Yadavalli Venkateswara, Neelamraju Sarla
National Professor Project, ICAR-Indian Institute of Rice Research, Hyderabad, India.
Department of Biotechnology, Acharya Nagarjuna University, Guntur, India.
Front Plant Sci. 2022 Mar 9;13:790221. doi: 10.3389/fpls.2022.790221. eCollection 2022.
Wild introgressions play a crucial role in crop improvement by transferring important novel alleles and broadening allelic diversity of cultivated germplasm. In this study, two stable backcross alien introgression lines 166s and 14s derived from Swarn/ IRGC81848 were used as parents to generate populations to map quantitative trait loci (QTLs) for yield-related traits. Field evaluation of yield-related traits in F, F, and F population was carried out in normal irrigated conditions during the wet season of 2015 and dry seasons of 2016 and 2018, respectively. Plant height, tiller number, productive tiller number, total dry matter, and harvest index showed a highly significant association to single plant yield in F, F, and F. In all, 21, 30, and 17 QTLs were identified in F, F, and F, respectively, for yield-related traits. QTLs with 12.54% phenotypic variance (PV) in F, with 13.01% PV, with 10.08% PV in F, and with 15.19% PV in F were identified as major effect QTLs. QTLs and were detected for grain yield in F and F with PV 8.5 and 6.7%, respectively. The trait enhancing alleles of QTLs , , , , and were from . QTLs of the yield contributing traits were found clustered in the same chromosomal region. was identified in a 2.6 Mb region between RM3480 and RM3452 in all three generations with PV 6.1 to 9.8%. This stable and consistent allele from can be fine mapped for identification of causal genes. From this population, lines C12, C124, C128, and C143 were identified with significantly higher SPY and C103, C116, and C117 had consistently higher thousand-grain weight values than both the parents and Swarna across the generations and are useful in gene discovery for target traits and further crop improvement.
野生种质渗入通过转移重要的新等位基因和拓宽栽培种质的等位基因多样性，在作物改良中发挥着关键作用。在本研究中，以源自Swarn/IRGC81848的两个稳定回交异源渗入系166s和14s作为亲本构建群体，用于定位产量相关性状的数量性状位点（QTL）。分别于2015年雨季正常灌溉条件下以及2016年和2018年旱季对F1、F2和F3群体的产量相关性状进行田间评估。株高、分蘖数、有效分蘖数、总干物质和收获指数在F1、F2和F3中与单株产量均表现出极显著关联。在F1、F2和F3中分别鉴定出21个、30个和17个与产量相关性状的QTL。在F1中具有12.54%表型变异（PV）、F2中具有13.01% PV、F3中具有10.08% PV以及F3中具有15.19% PV的QTL被鉴定为主要效应QTL。在F2和F3中检测到与籽粒产量相关的QTL，其PV分别为8.5%和6.7%。QTL qYLD2.1、qYLD2.2、qYLD3.1、qYLD3.2和qYLD3.3的性状增强等位基因来自Swarn。产量贡献性状的QTL在同一染色体区域成簇分布。在所有三个世代中，qYLD3.1在RM3480和RM3452之间的2.6 Mb区域被鉴定出来，其PV为6.1%至9.8%。来自Swarn的这个稳定且一致的等位基因可以进行精细定位以鉴定因果基因。从该群体中，鉴定出C12、C124、C128和C143株系的单株产量显著更高，C103、C116和C117在各世代中的千粒重始终高于双亲，这些株系可用于目标性状的基因发现和进一步的作物改良。
