He Lijuan, Wang Zhaokai, Lou Sulin, Lin Xiangzhi, Hu Fan
Xiamen Overseas Chinese Subtropical Plant Introduction Garden, Xiamen, Fujian, China.
Engineering Research Center of Marine Biological Resource Comprehensive Utilization, Third Institute of Oceanography, State Oceanic Administration, Xiamen, Fujian, China.
Genes Genomics. 2018 May;40(5):543-552. doi: 10.1007/s13258-018-0652-x. Epub 2018 Feb 1.
In this study, the chloroplast genome of Hariotina reticulata was fully sequenced and compared to other Sphaeropleales chloroplast genomes. It is 210,757 bp larger than most Sphaeropleales cpDNAs. It presents a traditional chloroplast structure, and contains 103 genes, including 68 protein-coding genes, six rRNA genes and 29 tRNA genes. The coding region constitutes of 43% of the whole cpDNA. Eighteen introns are found in 11 genes and six introns are unique for Hariotina. 11 open reading frames are identified among these introns. The synteny between Hariotina and Acutodesmus cpDNAs is in general identical, while within Sphaeropleales order, high variability in cpDNA architecture is indicated by general high DCJ distances. Ankyra judayi exhibits the greatest dissimilarity in gene synteny to the others and share some unique gene clusters with Treubaria triappendiculata. The phylogenomic analyses show that A. judayi is clustered with Treubariaceae species and sister to Chlorophyceae incertae sedis and other Sphaeropleales species. The monophyly of Sphaeropleales is rejected.
在本研究中，对网纹哈里奥藻（Hariotina reticulata）的叶绿体基因组进行了全测序，并与其他小球藻目叶绿体基因组进行了比较。它比大多数小球藻目叶绿体DNA大210,757碱基对。它呈现出传统的叶绿体结构，包含103个基因，其中包括68个蛋白质编码基因、6个rRNA基因和29个tRNA基因。编码区占整个叶绿体DNA的43%。在11个基因中发现了18个内含子，其中6个内含子是哈里奥藻特有的。在这些内含子中鉴定出11个开放阅读框。哈里奥藻和尖针藻属叶绿体DNA之间的同线性总体上是相同的，而在小球藻目内，叶绿体DNA结构的高度变异性由普遍较高的DCJ距离表明。尤氏锚形藻（Ankyra judayi）在基因同线性上与其他物种表现出最大的差异，并与三叉三棒藻（Treubaria triappendiculata）共享一些独特的基因簇。系统基因组分析表明，尤氏锚形藻与三棒藻科物种聚类，是绿藻纲不确定分类地位物种和其他小球藻目物种的姐妹群。小球藻目的单系性被否定。
