Biochemistry Department, LR12SP11, Sahloul University Hospital, Sousse, Tunisia.
Laboratory of Biochemistry and Toxicology, Monastir's University Hospital, Monastir, Tunisia.
Int J Exp Pathol. 2018 Jun;99(3):121-130. doi: 10.1111/iep.12271. Epub 2018 Jun 11.
Adipose tissue is an important endocrine organ that secretes a number of adipokines, like Leptin (LEP). The aim this study was to investigate the prevalence of single nucleotide polymorphisms in LEP gene (LEP 3'UTR A/C, -2548 G/A) and LEPR (K109R and Q223R) and their association with Leptin level and obesity. We recruited 169 non-obese (body mass index [BMI] = 24.51-3.69 kg/m ) and 160 obese (BMI = 36-4.78 kg/m ) patients. Genotyping was performed using polymerase chain reaction-restriction fragment length polymorphism, BMI was calculated, and Leptin level was measured by ELISA. Statistical analyses were performed by spss19.0. According to LEP 3'UTR A/C polymorphism, AC and CC genotype carriers had higher Leptin levels than AA genotype carriers, respectively, 31[0.05-148.8] (P = .008) vs 41[0.05-111.6] (P = .003). The K109R polymorphism was associated with obesity (P = .025) and seems to significantly decrease the LEP levels (P < .001). Concerning LEP G2548A polymorphism, our results showed that the OR of obesity associated with 2548 AA/GG was 1.87[1.106-2.78] P = .028 vs 1.41[1.035-1.85] P = .045 for 223AA/GG polymorphism. In our haplotype analysis, one haplotype seems to be the more protective and one other seems to be the highest risk to obesity. LEP 3'UTR A/C and LEPR K109R polymorphisms were associated with Leptin level and obesity.
脂肪组织是一个重要的内分泌器官，它分泌许多脂肪因子，如瘦素（LEP）。本研究旨在探讨 LEP 基因（LEP 3'UTR A/C、-2548 G/A）和 LEPR（K109R 和 Q223R）单核苷酸多态性的流行率及其与瘦素水平和肥胖的关系。我们招募了 169 名非肥胖（体重指数 [BMI] = 24.51-3.69 kg/m²）和 160 名肥胖（BMI = 36-4.78 kg/m²）患者。使用聚合酶链反应-限制性片段长度多态性进行基因分型，计算 BMI，并通过 ELISA 测量瘦素水平。统计分析采用 spss19.0 进行。根据 LEP 3'UTR A/C 多态性，AC 和 CC 基因型携带者的瘦素水平高于 AA 基因型携带者，分别为 31[0.05-148.8]（P = 0.008）和 41[0.05-111.6]（P = 0.003）。K109R 多态性与肥胖有关（P = 0.025），似乎显著降低 LEP 水平（P < 0.001）。关于 LEP G2548A 多态性，我们的结果表明，与 2548 AA/GG 相关的肥胖 OR 为 1.87[1.106-2.78] P = 0.028，与 223AA/GG 多态性的 1.41[1.035-1.85] P = 0.045。在我们的单体型分析中，一种单体型似乎是保护性的，另一种单体型似乎是肥胖的最高风险。LEP 3'UTR A/C 和 LEPR K109R 多态性与瘦素水平和肥胖有关。
