Zayani Nesrine, Hamdouni Haithem, Boumaiza Imen, Achour Ons, Neffati Fadoua, Omezzine Asma, Najjar Mohamed Fadhel, Bouslama Ali
Biochemistry Department, Sahloul University Hospital, Sousse, Tunisia.
Laboratory of Biochemistry and Toxicology, Monastir's University Hospital, Monastir, Tunisia.
J Clin Lab Anal. 2018 Feb;32(2). doi: 10.1002/jcla.22227. Epub 2017 Apr 10.
Adipose tissue is an important endocrine organ that secretes a number of adipokines, like Resistin (RETN); it's an adipocytes-secreted cytokine and has been proposed as a link between obesity and diabetes. Many resistin gene polymorphisms were described and their implication in obesity was controversial. This study was to investigate the prevalence of single nucleotide polymorphisms (SNPs) in RETN gene 420C/G; 44G/A; 62G/A; 394C/G and 299 G/A and their association with Resistin level and obesity in Tunisian volunteers.
We recruited 169 nonobese (mean age=42.16-14.26 years; mean body mass index [BMI]=24.51-3.69 kg/m ) and 160 obese (mean age=47.86-11.17 years; mean BMI=36-4.78 kg/m ). Genotyping was performed using polymerase chain reaction-restriction fragment length polymorphism. Anthropometric parameters, lipid levels, Glycemia and insulinemia were measured, BMI was calculated and insulinresistance was evaluated with the homeostasis model assessment insulin resistance (HOMA-IR) and resistin level was measured by ELISA. Statistical analyses were performed by SPSS19.0.
After adjustment for confounding parameters; the Odds Ratio (OR) of obesity associated with mutated genotypes at 420C/G compared with normal genotype was as: OR=2.17; 95% CI [1.28-3.68], P=.004. The serum Resistin levels present no significant association with all RETN polymorphisms and it was significantly associated with BMI (P=.047). In our haplotype analysis, one haplotype seems to be protective and one other seems to be the highest risk to obesity.
The 420 C/G Polymorphism were associated with obesity and Leptin concentration in our population.
脂肪组织是一个重要的内分泌器官，可分泌多种脂肪因子，如抵抗素（RETN）；它是一种由脂肪细胞分泌的细胞因子，被认为是肥胖与糖尿病之间的一个联系环节。许多抵抗素基因多态性已被描述，但其与肥胖的关系存在争议。本研究旨在调查突尼斯志愿者中RETN基因420C/G、44G/A、62G/A、394C/G和299G/A单核苷酸多态性（SNP）的发生率及其与抵抗素水平和肥胖的关联。
我们招募了169名非肥胖者（平均年龄=42.16 - 14.26岁；平均体重指数[BMI]=24.51 - 3.69kg/m²）和160名肥胖者（平均年龄=47.86 - 11.17岁；平均BMI=36 - 4.78kg/m²）。采用聚合酶链反应-限制性片段长度多态性进行基因分型。测量人体测量参数、血脂水平、血糖和胰岛素血症，计算BMI，并使用稳态模型评估胰岛素抵抗（HOMA-IR）评估胰岛素抵抗，通过酶联免疫吸附测定法测量抵抗素水平。使用SPSS19.0进行统计分析。
在对混杂参数进行调整后；与正常基因型相比，420C/G位点突变基因型与肥胖相关的优势比（OR）为：OR = 2.17；95%置信区间[1.28 - 3.68]，P = 0.004。血清抵抗素水平与所有RETN多态性均无显著关联，而与BMI显著相关（P = 0.047）。在我们的单倍型分析中，一种单倍型似乎具有保护作用，而另一种似乎是肥胖的最高风险因素。
在我们的人群中，420C/G多态性与肥胖和瘦素浓度相关。
