解淀粉芽孢杆菌产3-羟基丁酮的突变及发酵优化
[Mutation and fermentation optimization of Bacillus amyloliquefaciens for acetoin production].
作者信息
Wang Shihui, Luo Qiuling, Liu Jia, Liu Liming, Chen Xiulai
机构信息
State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, Jiangsu, China.
Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, Wuxi 214122, Jiangsu, China.
出版信息
Sheng Wu Gong Cheng Xue Bao. 2018 May 25;34(5):803-811. doi: 10.13345/j.cjb.170446.
As a platform chemical, acetoin has a great potential of application in medicine and food industries. In order to improve the efficiency of acetoin production, Bacillus amyloliquefaciens was treated by atmospheric and room temperature plasma and gamma rays. Two-round screening was adopted for obtaining positive mutants, and the best mutant B. amyloliquefaciens H-5 produced acetoin up to 68.2 g/L in shake flask. Then, culture conditions were optimized in 5-L fermentor to enhance acetoin production. Finally, 85.2 g/L acetoin was produced by B. amyloliquefaciens H-5, which was increased by 26.8% compared with that of the original strain B. amyloliquefaciens FMME088. These results indicated that the high-producing strain can be obtained efficiently by compound mutagenesis, which has a promising prospect for commercial scale process.
作为一种平台化学品，3-羟基丁酮在医药和食品工业中具有巨大的应用潜力。为了提高3-羟基丁酮的生产效率，对解淀粉芽孢杆菌进行了常压室温等离子体和γ射线处理。采用两轮筛选获得阳性突变体，最佳突变体解淀粉芽孢杆菌H-5在摇瓶中3-羟基丁酮产量高达68.2 g/L。然后，在5-L发酵罐中优化培养条件以提高3-羟基丁酮产量。最终，解淀粉芽孢杆菌H-5生产的3-羟基丁酮为85.2 g/L，与原始菌株解淀粉芽孢杆菌FMME088相比提高了26.8%。这些结果表明，通过复合诱变可以高效获得高产菌株，在商业规模生产中具有广阔的前景。
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