State Key Laboratory of Food Science and Technology, Jiangnan University, 1800 Lihu Road, Wuxi 214122, Jiangsu Province, China.
Bioresour Technol. 2013 Feb;130:256-60. doi: 10.1016/j.biortech.2012.10.036. Epub 2012 Oct 18.
A new strain, FMME044, exhibited a remarkable ability to synthesize acetoin and was identified as Bacillus amyloliquefaciens. The following characteristics of enzyme activity were found: 2,3-butanediol was reverse transformed to acetoin upon depletion of glucose; lower agitation speeds favored 2,3-butanediol accumulation; and higher agitation speeds favored reverse transformation of 2,3-butanediol to acetoin. In order to enhance acetoin production by manipulating the carbon flux distribution, a two-stage agitation speed control strategy was proposed: during the first 24h, the agitation speed was set to 350rpm to achieve a high 2,3-butanediol concentration and then the speed was increased to 500rpm to reverse transform 2,3-butanediol to acetoin. Following this strategy, a high titer (51.2gL(-1)), yield (0.43gg(-1)), and productivity (1.42gL(-1)h(-1)) of acetoin were achieved. The results demonstrated that B. amyloliquefaciens FMME044 is a potential industrial strain for acetoin production.
一种新的菌株 FMME044 表现出显著的合成乙酰的能力，并被鉴定为解淀粉芽孢杆菌。该酶的活性具有以下特点：葡萄糖耗尽时，2,3-丁二醇被逆向转化为乙酰；较低的搅拌速度有利于 2,3-丁二醇的积累；而较高的搅拌速度有利于 2,3-丁二醇的逆向转化为乙酰**。为了通过操纵碳通量分布来提高乙酰的产量，提出了一种两阶段搅拌速度控制策略：在前 24 小时内，将搅拌速度设置为 350rpm 以达到较高的 2,3-丁二醇浓度，然后将速度提高到 500rpm 以将 2,3-丁二醇逆向转化为乙酰。采用该策略，获得了较高的乙酰浓度（51.2gL(-1)）、产率（0.43gg(-1)）和生产强度（1.42gL(-1)h(-1)）。结果表明，解淀粉芽孢杆菌 FMME044 是一种具有生产乙酰潜力的工业菌株。
