DeCherney G S, DeBold C R, Jackson R V, Sheldon W R, Island D P, Orth D N
J Clin Endocrinol Metab. 1985 Aug;61(2):273-9. doi: 10.1210/jcem-61-2-273.
To determine whether the plasma immunoreactive ACTH (IR-ACTH) and IR-cortisol responses to ovine corticotropin-releasing hormone (oCRH) depend on the time of day, we administered 1 microgram/kg BW synthetic oCRH as an iv bolus dose to five normal men at their usual time of awakening between 0530-0740 h, at 1600 h, and at 2300 h. Mean basal plasma IR-ACTH and IR-cortisol levels were highest upon awakening, intermediate at 1600 h, and lowest at 2300 h, reflecting the diurnal rhythm of ACTH secretion. There was no significant difference in the plasma IR-ACTH response to oCRH at different times of the day. In contrast, the mean maximum plasma IR-cortisol increment and mean integrated response were 2- and 2.6-fold greater (P less than 0.05), respectively, at 2300 h than upon awakening. In another study, oCRH was given in the morning (0700-0900 h) to 22 normal men and in the late afternoon (1600-1800 h) to 24 normal men. Mean basal plasma IR-ACTH and IR-cortisol levels were significantly higher (P less than 0.001) in the morning [24 +/- 3 pg/ml (mean +/- SEM) and 10.6 +/- 0.8 micrograms/dl, respectively] than in the afternoon (13 +/- 2 pg/ml and 5.6 +/- 0.6 micrograms/dl, respectively). Mean peak plasma IR-ACTH was slightly greater in the morning (60 +/- 5.5 pg/ml) than in the afternoon (47 +/- 5.5 pg/ml), the mean maximum plasma IR-ACTH increments were the same (35 +/- 4 and 34 +/- 5 pg/ml, respectively), and the mean integrated IR-ACTH response was slightly less in the morning (2036 +/- 414 vs. 2365 +/- 358 pg . min/ml), but none of these differences was statistically significant. Mean peak plasma IR-cortisol concentrations in the morning and afternoon were similar (18.7 +/- 0.7 and 17.3 +/- 0.9 micrograms/dl, respectively), but the mean maximum plasma IR-cortisol increments (8.1 +/- 0.8 and 11.7 +/- 0.9 micrograms/dl, respectively; P less than 0.005), and the mean integrated IR-cortisol responses (588 +/- 115 and 976 +/- 95 micrograms . min/dl, respectively; P less than 0.01) were greater in the afternoon. There was an inverse correlation between basal plasma IR-cortisol concentration and the integrated IR-ACTH response (P less than 0.05), the maximum IR-cortisol increment (P less than 0.001), and the integrated IR-cortisol response (P less than 0.001).(ABSTRACT TRUNCATED AT 400 WORDS)
为了确定血浆免疫反应性促肾上腺皮质激素（IR-ACTH）和IR-皮质醇对羊促肾上腺皮质激素释放激素（oCRH）的反应是否取决于一天中的时间，我们在0530 - 0740时之间、1600时和2300时这三个时间点，按照1微克/千克体重的剂量静脉推注合成oCRH，对五名正常男性进行给药。平均基础血浆IR-ACTH和IR-皮质醇水平在醒来时最高，在1600时处于中间水平，在2300时最低，这反映了ACTH分泌的昼夜节律。一天中不同时间对oCRH的血浆IR-ACTH反应没有显著差异。相比之下，2300时的平均最大血浆IR-皮质醇增量和平均综合反应分别比醒来时高2倍和2.6倍（P < 0.05）。在另一项研究中，上午（0700 - 0900时）对22名正常男性给予oCRH，下午（1600 - 1800时）对24名正常男性给予oCRH。上午的平均基础血浆IR-ACTH和IR-皮质醇水平显著高于下午（P < 0.001），上午分别为[24 ± 3皮克/毫升（平均值 ± 标准误）和10.6 ± 0.8微克/分升]，下午分别为（13 ± 2皮克/毫升和5.6 ± 0.6微克/分升）。上午的平均血浆IR-ACTH峰值略高于下午（60 ± 5.5皮克/毫升比47 ± 5.5皮克/毫升），平均最大血浆IR-ACTH增量相同（分别为35 ± 4和34 ± 5皮克/毫升），上午的平均综合IR-ACTH反应略低于下午（2036 ± 414对2365 ± 358皮克·分钟/毫升），但这些差异均无统计学意义。上午和下午的平均血浆IR-皮质醇峰值浓度相似（分别为18.7 ± 0.7和17.3 ± 0.9微克/分升），但下午的平均最大血浆IR-皮质醇增量（分别为8.1 ± 0.8和11.7 ± 0.9微克/分升；P < 0.005）以及平均综合IR-皮质醇反应（分别为588 ± 115和976 ± 95微克·分钟/分升；P < 0.01）更大。基础血浆IR-皮质醇浓度与综合IR-ACTH反应（P < 0.05）、最大IR-皮质醇增量（P < 0.001）以及综合IR-皮质醇反应（P < 0.001）之间存在负相关。（摘要截短于400字）
