Plewka Danuta, Plewka Andrzej, Miskiewicz Adam, Morek Michał, Bogunia Edyta
Department of Cytophysiology, Chair of Histology and Embryology, Medical University of Silesia, Medyków 18, Katowice, Poland.
Department of Proteomics, Medical University of Silesia, 41-200 Sosnowiec, Poland.
J Cancer Res Ther. 2018 Apr-Jun;14(3):516-520. doi: 10.4103/0973-1482.180607.
It has been proved that nuclear factor-kappa B (NF-κB) is activated in all cells, promotes proliferation of cells, regulates the immunological and inflammatory response, and contribute to the pathogenesis of many conditions, including cancer. Many studies pointed to constitutive activation of NF-κB in cells of certain malignant tumors.
The aim of the study was to analyze the role of nuclear growth factor κB as colon cancer marker and prognostic factor.
The study included 59 primary colorectal tumor patients and 15 patients in control group. The tumor samples were taken during partial colectomy and colonoscopy in control group. Tissues samples were fixed and embedded in paraffin blocks and cut. Sections were used for schedule immunohistochemical staining with the application of specific antibody for NF-κB epitope. The marker expression was compared with well-known prognostic factors in colon tumors such as tumor type, stage, and grade to establish if it might be a potential prognostic factor.
The results showed statistically significant difference between control group and cancer group.
The expression NF-κB did not depend on the stage and grade of colon tumors.
已证实核因子-κB（NF-κB）在所有细胞中均被激活，可促进细胞增殖，调节免疫和炎症反应，并参与包括癌症在内的多种疾病的发病机制。许多研究指出某些恶性肿瘤细胞中NF-κB存在组成性激活。
本研究旨在分析核生长因子κB作为结肠癌标志物和预后因素的作用。
本研究纳入59例原发性结直肠癌患者和15例对照组患者。肿瘤样本在对照组的部分结肠切除术和结肠镜检查期间采集。组织样本固定后包埋于石蜡块中并切片。切片用于采用针对NF-κB表位的特异性抗体进行常规免疫组织化学染色。将该标志物表达与结肠肿瘤中众所周知的预后因素如肿瘤类型、分期和分级进行比较，以确定其是否可能是一个潜在的预后因素。
结果显示对照组和癌症组之间存在统计学显著差异。
NF-κB的表达不依赖于结肠肿瘤的分期和分级。
