Department of Food Science and Technology, Faculty of Nutrition Science and Food Technology, National Nutrition and Food Technology Research Institute, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Toxicology Research Center, Medical Basic Sciences Research Institute, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
Probiotics Antimicrob Proteins. 2024 Aug;16(4):1348-1364. doi: 10.1007/s12602-023-10117-y. Epub 2023 Jul 4.
A new biotherapeutic strategy involves the use of microbial bioactive substances (postbiotics) that exhibit optimum compatibility and intimate contact with the immune system of the host. This study was aimed at investigating the potential biological activities of postbiotics derived from Saccharomyces cerevisiae (PTCC 5269) (PSC) under in vitro circumstances. Based on the outcomes, the synthesized PSC possessing a high level of phenolic (102.46 ± 0.25 mg GAE/g) and flavonoid (19.87 ± 75.32 mg QE/g) content demonstrated significant radical scavenging activity (87.34 ± 0.56%); antibacterial action towards Listeria monocytogenes, Streptococcus mutans, Salmonella typhi, and Escherichia coli (in order of effectiveness) in both in vitro and food models (whole milk and ground meat); probiotics' growth-promoting activity in the fermentation medium; α-glucosidase enzyme-inhibiting and cholesterol-lowering properties in a concentration- and pH-dependent manner; reduction in the cell viability (with the significant IC values of 34.27 and 23.58 μg/mL after 24 and 48 h, respectively); suppressed the initial (G0/G1) phase of the cell's division; induced apoptosis; and increased the expression of PTEN gene, while the IkB, RelA, and Bcl-XL genes indicated diminished expression in treated SW480 cancer cells. These multiple health-promoting functions of PSC can be extended to medical, biomedical, and food scopes, as novel biotherapeutic approaches, in order to design efficient and optimized functional food formulations or/and supplementary medications to use as adjuvant agents for preventing or/and treating chronic/acute disorders.
一种新的生物治疗策略涉及使用微生物生物活性物质（后生元），这些物质与宿主的免疫系统具有最佳的相容性和密切接触。本研究旨在研究来源于酿酒酵母（PTCC 5269）（PSC）的后生元在体外环境下的潜在生物学活性。基于研究结果，合成的 PSC 具有高水平的酚（102.46±0.25mg GAE/g）和类黄酮（19.87±75.32mg QE/g）含量，表现出显著的自由基清除活性（87.34±0.56%）；在体外和食品模型（全脂牛奶和碎肉）中对李斯特菌、变形链球菌、伤寒沙门氏菌和大肠杆菌具有抗菌作用（按效果强弱排序）；在发酵培养基中促进益生菌的生长；在浓度和 pH 依赖性方式下具有α-葡萄糖苷酶抑制和降胆固醇作用；降低细胞活力（分别在 24 和 48 小时后具有显著的 IC 值 34.27 和 23.58μg/mL）；抑制细胞分裂的初始（G0/G1）阶段；诱导细胞凋亡；并增加 PTEN 基因的表达，而 IkB、RelA 和 Bcl-XL 基因在经处理的 SW480 癌细胞中的表达则减少。PSC 的这些多种促进健康的功能可扩展到医学、生物医学和食品领域，作为新的生物治疗方法，以设计高效和优化的功能性食品配方或/和补充药物，作为预防或/和治疗慢性/急性疾病的辅助剂。
