Department of Pathology, Massachusetts General Hospital, Boston.
Department of Pathology, Mbarara University of Science and Technology, Mbarara, Uganda.
Am J Clin Pathol. 2018 Jul 3;150(2):137-145. doi: 10.1093/ajcp/aqy031.
The plasma-thrombin method is commonly used to make cell blocks from fine-needle aspiration (FNA) samples but requires centrifugation. We describe a modification to this method that does not require centrifugation for use in resource-limited settings.
Pooled fresh plasma is aliquoted into 2-mL Eppendorf tubes and the FNA sample directly rinsed into the plasma. Two drops of reconstituted thrombin are added and gently mixed. A cell clot is transferred to a tissue bag, fixed in formalin, and processed. This method was applied to FNA samples from 44 patients presenting to the Mbarara University of Science and Technology FNA clinic.
The cell blocks were less cellular than the smears but contained adequate material to confirm morphologic impression or perform immunocytochemistry in 36 of 44 cases (82% adequacy rate).
The modified plasma-thrombin method is a reliable cell block preparation method that can be easily applied in resource-limited settings.
血浆-凝血酶法常用于从细针抽吸（FNA）样本中制作细胞块，但需要离心。我们描述了一种不需要离心的改良方法，以便在资源有限的环境中使用。
将 pooled fresh plasma 等分至 2-mL Eppendorf 管中，将 FNA 样本直接冲洗到血浆中。加入两滴再悬浮的凝血酶并轻轻混合。将细胞凝块转移到组织袋中，用福尔马林固定，然后进行处理。该方法应用于来自 44 名就诊于 Mbarara 科技大学 FNA 诊所的患者的 FNA 样本。
细胞块的细胞数量少于涂片，但足以确认形态印象或在 44 例中的 36 例（82%的充分性率）中进行免疫细胞化学。
改良的血浆-凝血酶法是一种可靠的细胞块制备方法，可在资源有限的环境中轻松应用。
