Villarreal L P, Soo N J
J Mol Appl Genet. 1985;3(1):62-71.
Transient expression of an SV40-based shuttle vector similar in design to commonly used vectors is shown to be inefficient when compared to expression of SV40 viral DNA. To eliminate this problem, we have designed and constructed a new vector, pSVPiC, which contains a minimal noninterfering plasmid component, the 885-bp plasmid PiAN7, and two SV40 promoter/origin regions. Transient expression of the SV40 late region from pSVPiC is much more efficient than that from previously used vectors and even more efficient than that from SV40 viral DNA. When the gene for rabbit beta-globin is placed in the late region of pSVPiC, it is also expressed at high levels, indicating that this shuttle vector is generally useful for expressing eukaryotic genes.
与常用载体设计相似的基于SV40的穿梭载体的瞬时表达，与SV40病毒DNA的表达相比效率较低。为消除这一问题，我们设计并构建了一种新载体pSVPiC，它包含一个最小的非干扰质粒成分，即885碱基对的质粒PiAN7，以及两个SV40启动子/起始区域。来自pSVPiC的SV40晚期区域的瞬时表达比以前使用的载体更有效，甚至比来自SV40病毒DNA的表达更有效。当兔β-珠蛋白基因置于pSVPiC的晚期区域时，它也能高水平表达，这表明这种穿梭载体通常可用于表达真核基因。
