Shandong Key Laboratory of TCM Quality Control Technology, Shandong Analysis and Test Center, Qilu University of Technology (Shandong Academy of Sciences), Jinan, 250014, PR China.
Jinan Senkang Sanfeng Biological Engineering Technology Co. Ltd., Jinan, 250014, PR China.
Fitoterapia. 2018 Sep;129:54-61. doi: 10.1016/j.fitote.2018.06.006. Epub 2018 Jun 9.
Two new alkaloid glycosides (1-2), two new amide alkaloids (3 and 6), and two new amine alkaloids (4 and 5), were isolated from Scindapsus officinalis (Roxb.) Schott., along with four known alkaloids (7-10). Structural elucidation of the isolated compounds was established by spectroscopic analysis, especially 2D NMR techniques and comparison with literatures. The absolute configurations of compounds 1 and 2 were determined by the electronic circular dichroism calculations. Compound 1 represent the first example of alkaloid glycoside found to possess an unprecedented 2,5-methanocyclopenta[b]azepine core. All isolates were evaluated for cytotoxic activity against MCF-7 human breast cancer cell and anti-inflammatory activity against nitric oxide (NO) production in vitro. Compounds 4, 6 and 8 demonstrated moderate cytotoxic effects against MCF-7 cell (IC of 11.6, 25.3, and 20.5 μM, respectively), and 1, 7 and 9 exhibited moderate inhibitory effects on NO production (IC of 19.8, 30.3, and 11.8 μM, respectively).
从 Scindapsus officinalis (Roxb.) Schott. 中分离得到了两个新的生物碱糖苷（1-2）、两个新的酰胺生物碱（3 和 6）和两个新的胺生物碱（4 和 5），以及四个已知的生物碱（7-10）。通过光谱分析，特别是二维 NMR 技术和与文献的比较，确定了分离化合物的结构。通过电子圆二色谱计算确定了化合物 1 和 2 的绝对构型。化合物 1 代表了首次发现具有前所未有的 2,5-甲烷环戊并[b]氮杂卓核心的生物碱糖苷。所有分离物均对 MCF-7 人乳腺癌细胞的细胞毒性活性和体外一氧化氮（NO）产生的抗炎活性进行了评估。化合物 4、6 和 8 对 MCF-7 细胞具有中等的细胞毒性作用（IC 分别为 11.6、25.3 和 20.5µM），化合物 1、7 和 9 对 NO 产生具有中等的抑制作用（IC 分别为 19.8、30.3 和 11.8µM）。
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