Department of Clinical Veterinary Medicine, College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi 712100, PR China.
Department of Veterinary Surgery, College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, PR China.
Int J Biol Macromol. 2018 Oct 1;117:1211-1215. doi: 10.1016/j.ijbiomac.2018.06.039. Epub 2018 Jun 9.
Zoletil is an anesthetic and immobilizing drug that has been used in the veterinary field for over 50 years; however, the effect of Zoletil, or its constituents, on brain cystathionine β-synthase (CBS) remains unknown. Here, we aimed to determine the effect of Zoletil on rat brain CBS by administering a single intraperitoneal injection of the drug and examining hydrogen sulfide (HS) and CBS levels in the cerebral cortex, hippocampus, and thalamus following three distinct behavioral phenotypes associated with the sedation procedure (e.g., loss of the righting reflex, return of the righting reflex, and return of walking). Zoletil administration resulted in significant decreases of endogenous HS in the cerebral cortex, hippocampus, and thalamus, and HS was observed to increase in these brain regions when rats recovered from the anesthesia. Quantitative real-time polymerase chain reaction, western blot, and immunohistochemistry revealed that CBS expression in the cerebral cortex and hippocampus exhibited the same trend as endogenous HS following Zoletil administration. In summary, our results demonstrated that Zoletil induced the expression of CBS which could exert region-specific regulation of HS in the cerebral cortex and hippocampus.
佐莱托是一种麻醉和固定药物，在兽医领域已经使用了 50 多年；然而，佐莱托或其成分对脑胱硫醚 β-合酶（CBS）的影响尚不清楚。在这里，我们通过单次腹腔注射该药物，旨在确定佐莱托对大鼠大脑 CBS 的影响，并在与镇静程序相关的三种不同行为表型（例如，翻正反射丧失、翻正反射恢复和行走恢复）后，检查大脑皮层、海马体和丘脑中的硫化氢（HS）和 CBS 水平。佐莱托给药导致大脑皮层、海马体和丘脑内源性 HS 显著降低，当大鼠从麻醉中恢复时，观察到这些脑区的 HS 增加。实时定量聚合酶链反应、western blot 和免疫组织化学显示，佐莱托给药后，CBS 在大脑皮层和海马体中的表达与内源性 HS 呈现相同趋势。总之，我们的结果表明，佐莱托诱导了 CBS 的表达，这可能对大脑皮层和海马体中的 HS 产生特定区域的调节。
