Astashev M E, Serov D A, Tankanag A V
Institute of Cell Biophysics, Russian Academy of Science, Pushchino, Moscow region, Russia.
Institute of Fundamental Problems of Biology, Russian Academy of Science, Pushchino, Moscow region, Russia.
Skin Res Technol. 2019 Jan;25(1):40-46. doi: 10.1111/srt.12593. Epub 2018 May 23.
When laboratory animals are used one needs to anesthetize them before recording. However, the influence of anesthesia on animal blood flow oscillations has not been studied. The effects of two ways of anesthesia, zoletil-xylazine, and zoletil-nitrous oxide mixtures, on mouse skin perfusion using laser Doppler flowmetry (LDF) technique were studied.
BALB/c mice were used. LDF probe was placed on the ventral surface of the left hind paw. Spectral analysis of LDF signals was performed with continuous adaptive wavelet transform to identify and describe peripheral blood flow oscillations in mouse skin.
Low-frequency oscillation interval boundaries (myogenic, neurogenic, and endothelial) for mice were shown to coincide with the boundaries determined for human and rats, that demonstrate their independence from the body size. Zoletil-xylazine anesthesia significantly decreased neurogenic and endothelial oscillation amplitudes by 29% and 50% respectively and increased the amplitude of cardiac oscillations by 23% compared to zoletyl-nitrous oxide anesthesia. There were no significant changes of the amplitudes of myogenic and respiratory oscillations with zoletil-nitrous oxide anesthesia compared to the zoletil-xylazine mixture.
We suggest that the different influence of anesthesia modes on the amplitudes of skin blood flow oscillations is associated with sympathetic activity suppressed by zoletil-xylazine anesthesia.
在使用实验动物时，需要在记录前对其进行麻醉。然而，麻醉对动物血流振荡的影响尚未得到研究。本研究采用激光多普勒血流仪（LDF）技术，探讨了两种麻醉方式（佐乐定-赛拉嗪和佐乐定-一氧化二氮混合物）对小鼠皮肤灌注的影响。
使用BALB/c小鼠。将LDF探头置于左后爪腹面。采用连续自适应小波变换对LDF信号进行频谱分析，以识别和描述小鼠皮肤外周血流振荡。
小鼠的低频振荡间隔边界（肌源性、神经源性和内皮源性）与人及大鼠的边界一致，表明其与体型无关。与佐乐定-一氧化二氮麻醉相比，佐乐定-赛拉嗪麻醉使神经源性和内皮源性振荡幅度分别显著降低29%和50%，并使心脏振荡幅度增加23%。与佐乐定-赛拉嗪混合物相比，佐乐定-一氧化二氮麻醉下肌源性和呼吸性振荡幅度无显著变化。
我们认为，麻醉方式对皮肤血流振荡幅度的不同影响与佐乐定-赛拉嗪麻醉抑制交感神经活动有关。
