Key Laboratory on Luminescence and Real-Time Analysis, Ministry of Education, School of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, China.
Key Laboratory on Luminescence and Real-Time Analysis, Ministry of Education, School of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, China.
Spectrochim Acta A Mol Biomol Spectrosc. 2018 Oct 5;203:421-427. doi: 10.1016/j.saa.2018.05.078. Epub 2018 May 26.
In this paper, the high performance fluorescent carbon dots were synthesized with maleic acid, tris and benzoic acid as raw materials by one-step hydrothermal method. The obtained carbon dots with uniform size emitted strong blue fluorescence, which the maximum excitation and emission wavelengths at 250 nm and 415 nm, respectively. Under the optimum condition, it was meaningfully founded that the reaction between the carbon dots and uric acid resulting in the fluorescence quenching of the carbon dots at the emission spectrum of 415 nm. The reason was that they had a synergistic effect between the fluorescence internal filtering effect and the static quenching effect. The fluorescence internal filter effect sensing system was constructed by using uric acid as the absorbable material and carbon dots as the luminophore. Hence, a fluorescence quenching method for the determination of uric acid was established in the concentration range from 5.0 to 400 μM with the detection limit (3σ/S) of 2.26 μM. Thus, a fluorescent sensing assay for the determination of uric acid was founded and confirmed in human fluids.
本文以马来酸、三羟甲基丙烷和苯甲酸为原料，采用一步水热法合成了高性能荧光碳点。所得碳点粒径均匀，发射强蓝光，最大激发和发射波长分别为 250nm 和 415nm。在最佳条件下，发现碳点与尿酸之间的反应导致碳点在 415nm 的发射光谱处荧光猝灭。原因是它们之间存在荧光内滤效应和静态猝灭效应的协同作用。以尿酸为吸收材料，碳点为发光体，构建了荧光内滤效应传感体系。因此，建立了一种在 5.0 至 400μM 浓度范围内测定尿酸的荧光猝灭法，检测限（3σ/S）为 2.26μM。因此，建立并验证了一种用于测定人液中尿酸的荧光传感分析方法。
