Faculty of Life Science and Technology, Kunming University of Science and Technology, Yunnan Province 650500, China.
Civil and Environmental Engineering, Carnegie Mellon University, Pittsburgh, PA 15213-3890, United States.
Spectrochim Acta A Mol Biomol Spectrosc. 2018 Feb 5;190:360-367. doi: 10.1016/j.saa.2017.09.037. Epub 2017 Sep 15.
In this work, a green, simple, economical method was developed in the synthesis of fluorescent carbon dots using pork as carbon source. The as-prepared carbon dots exhibit exceptional advantages including high fluorescent quantum yield (17.3%) and satisfactory chemical stability. The fluorescence of carbon dots based nanosensor can be selectively and efficiently quenched by uric acid. This phenomenon was used to develop a fluorescent method for facile detection of uric acid within a linear range of 0.1-100μM and 100-500μM, with a detection limit of 0.05μM (S/N=3). Finally, the proposed method was successfully applied in the determination of uric acid in human serum and urine samples with satisfactory recoveries, which suggested that the new nanosensors have great prospect toward the detection of uric acid in human fluids.
在这项工作中,开发了一种使用猪肉作为碳源合成荧光碳点的绿色、简单、经济的方法。所制备的碳点具有高荧光量子产率(17.3%)和令人满意的化学稳定性等优异性能。基于碳点的纳米传感器的荧光可以被尿酸选择性和有效地猝灭。利用这一现象,开发了一种荧光法,用于在 0.1-100μM 和 100-500μM 的线性范围内简便检测尿酸,检测限为 0.05μM(S/N=3)。最后,该方法成功应用于人血清和尿液样品中尿酸的测定,回收率令人满意,这表明新型纳米传感器在人液中尿酸检测方面具有广阔的前景。