Pittet A C, Schibler U
J Mol Biol. 1985 Apr 5;182(3):359-65. doi: 10.1016/0022-2836(85)90196-2.
We have cloned a contiguous 106 X 10(3) base-pair long stretch of mouse DNA. The isolated chromosomal DNA segment contains the single copy gene Amy-1a that is strongly expressed in the parotid gland and, 23 X 10(3) base-pairs downstream from it, one member of the pancreas-specific Amy-2a oligogene family. At least two of the four Amy-2a genes, including the copy linked to Amy-1a, are efficiently transcribed. The cloned DNA sequences do not appear to specify messenger RNAs other than those encoding alpha-amylase in pancreas, parotid gland or liver. Transcription termination on Amy-1a occurs within 3 X 10(3) base-pairs downstream from the polyadenylation site in both parotid gland and liver, in which this gene is transcribed at different rates from different promoters.
我们克隆了一段连续的、长度为106×10³碱基对的小鼠DNA。分离出的染色体DNA片段包含在腮腺中强烈表达的单拷贝基因Amy-1a，以及在其下游23×10³碱基对处的胰腺特异性Amy-2a寡基因家族的一个成员。四个Amy-2a基因中至少有两个，包括与Amy-1a相连的拷贝，能被有效转录。克隆的DNA序列似乎除了在胰腺、腮腺或肝脏中编码α-淀粉酶的信使RNA外，并不指定其他信使RNA。Amy-1a的转录终止发生在腮腺和肝脏中聚腺苷酸化位点下游3×10³碱基对范围内，在腮腺和肝脏中，该基因从不同启动子以不同速率转录。
