Schibler U, Hagenbüchle O, Wellauer P K, Pittet A C
Cell. 1983 Jun;33(2):501-8. doi: 10.1016/0092-8674(83)90431-2.
We show that two promoters of different strengths are involved in the tissue-specific expression of the alpha-amylase gene Amy-1a in the parotid gland and the liver of mouse. The weaker of the two promoters directs the synthesis of mRNA with a liver-type leader sequence. This promoter is active in both tissues. A promoter that is about 30-fold stronger is exclusively active in the parotid, where it directs the synthesis of an mRNA with a parotid-specific leader sequence. Neither the parotid nor the liver promoter is used in tissues that do not contain cytoplasmic alpha-amylase mRNAs, such as brain, kidney, and spleen. Nuclear transcripts that are initiated several kilobases upstream of the parotid cap site are detected in several tissues. They are most abundant in brain, and are apparently not processed into alpha-amylase mRNA.
我们发现，不同强度的两个启动子参与了小鼠腮腺和肝脏中α-淀粉酶基因Amy-1a的组织特异性表达。两个启动子中较弱的那个指导具有肝脏型前导序列的mRNA的合成。该启动子在两种组织中均有活性。一个强度约强30倍的启动子仅在腮腺中具有活性，在腮腺中它指导具有腮腺特异性前导序列的mRNA的合成。腮腺和肝脏的启动子在不含有细胞质α-淀粉酶mRNA的组织（如脑、肾和脾）中均未被使用。在几个组织中检测到在腮腺帽位点上游几千个碱基处起始的核转录本。它们在脑中最为丰富，显然未被加工成α-淀粉酶mRNA。
