小鼠两组组蛋白基因的差异表达。

Differential expression of two clusters of mouse histone genes.

作者信息

Graves R A, Wellman S E, Chiu I M, Marzluff W F

出版信息

J Mol Biol. 1985 May 25;183(2):179-94. doi: 10.1016/0022-2836(85)90211-6.

Abstract

The mouse histone mRNAs coded for by three different cloned DNA fragments have been characterized. Two of these cloned DNA fragments, MM221 and MM291, located on chromosome 13, code for H3, H2b and H2a histone mRNAs, which are expressed at low levels in cultured mouse cells and fetal mice. The other DNA fragment, MM614, located on chromosome 3, codes for an H3 and an H2a mRNA, which are expressed at high levels in these cells. The mRNAs for each histone protein share common coding region sequences, while the untranslated regions of all the genes have diverged significantly, as judged by S1 nuclease mapping. Amino acid substitutions in some H3, H2a and H2b proteins are detected as internal cleavages in the S1 nuclease maps. All of these genes code for replication variant histone mRNAs, which are regulated in parallel with DNA synthesis.

摘要

由三个不同的克隆DNA片段编码的小鼠组蛋白mRNA已得到鉴定。其中两个克隆DNA片段，MM221和MM291，位于13号染色体上，编码H3、H2b和H2a组蛋白mRNA，它们在培养的小鼠细胞和胎鼠中低水平表达。另一个DNA片段MM614位于3号染色体上，编码一个H3和一个H2a mRNA，它们在这些细胞中高水平表达。每种组蛋白的mRNA共享共同的编码区序列，而根据S1核酸酶图谱判断，所有基因的非翻译区已经显著分化。在S1核酸酶图谱中，一些H3、H2a和H2b蛋白中的氨基酸取代被检测为内部切割。所有这些基因都编码复制变体组蛋白mRNA，其与DNA合成平行调节。