• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

禽成髓细胞瘤病毒前病毒DNA对来航鸡胚成纤维细胞的转化

Transformation of Brown Leghorn chicken embryo fibroblasts by avian myeloblastosis virus proviral DNA.

作者信息

Soret J, Kryceve-Martinerie C, Crochet J, Perbal B

出版信息

J Virol. 1985 Jul;55(1):193-205. doi: 10.1128/JVI.55.1.193-205.1985.

DOI:10.1128/JVI.55.1.193-205.1985
PMID:2989555
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC254915/
Abstract

Brown Leghorn chicken embryo fibroblasts were transfected with a mixture of avian myeloblastosis virus (AMV) and myeloblastosis-associated virus type 1 (MAV1) proviral DNA purified from lambda-Charon 4A recombinant clones. A transformed cell line (T1AM) able to grow without anchorage in semisolid medium was obtained. The presence of both proviral AMV and MAV sequences was detected in T1AM DNA by hybridization with v-myb- and MAV1-specific probes. Altered AMV and MAV1 proviral genomes were found in T1AM genome. Characterization of the RNA species expressed in transformed cells showed that in addition to a 2.5-kilobase (kb) putative subgenomic v-myb-specific RNA, three other myb-containing RNAs (9.4, 8.4, and 7.0 kb) were present in T1AM cells. No AMV genomic RNA was detected. Also, a new 5.0-kb MAV1-specific RNA species was expressed in transformed cells in addition to MAV1 genomic RNA species (7.8 kb). No infectious AMV virions are released by T1AM cells. Chicken embryo fibroblasts infected by T1AM-released virions contained and expressed all MAV1 sequences detected in T1AM transformed cells but did not express any transformation parameter. These results indicated that the presence of AMV proviral sequences in T1AM cells is responsible for their transformed phenotype.

摘要

用从λ-噬菌体4A重组克隆中纯化的禽成髓细胞瘤病毒（AMV）和1型成髓细胞瘤相关病毒（MAV1）前病毒DNA混合物转染白来航鸡胚胎成纤维细胞。获得了一种能够在半固体培养基中不依赖贴壁生长的转化细胞系（T1AM）。通过与v-myb和MAV1特异性探针杂交，在T1AM DNA中检测到前病毒AMV和MAV序列的存在。在T1AM基因组中发现了改变的AMV和MAV1前病毒基因组。对转化细胞中表达的RNA种类的表征表明，除了一种2.5千碱基（kb）的假定亚基因组v-myb特异性RNA外，T1AM细胞中还存在另外三种含myb的RNA（9.4、8.4和7.0 kb）。未检测到AMV基因组RNA。此外，除了MAV1基因组RNA种类（7.8 kb）外，转化细胞中还表达了一种新的5.0 kb MAV1特异性RNA种类。T1AM细胞不释放有感染性的AMV病毒粒子。被T1AM释放的病毒粒子感染的鸡胚胎成纤维细胞含有并表达了在T1AM转化细胞中检测到的所有MAV1序列，但不表达任何转化参数。这些结果表明，T1AM细胞中AMV前病毒序列的存在是其转化表型的原因。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1867/254915/eabb11ff1d9d/jvirol00118-0213-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1867/254915/593994aeae04/jvirol00118-0206-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1867/254915/bd9385e777bf/jvirol00118-0208-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1867/254915/5734c2fc65ba/jvirol00118-0208-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1867/254915/44b8a7ebae58/jvirol00118-0208-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1867/254915/676ece0cb3b8/jvirol00118-0209-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1867/254915/15d12fae2bd9/jvirol00118-0210-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1867/254915/c312298366ba/jvirol00118-0211-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1867/254915/466379eb11ea/jvirol00118-0212-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1867/254915/eabb11ff1d9d/jvirol00118-0213-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1867/254915/593994aeae04/jvirol00118-0206-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1867/254915/bd9385e777bf/jvirol00118-0208-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1867/254915/5734c2fc65ba/jvirol00118-0208-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1867/254915/44b8a7ebae58/jvirol00118-0208-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1867/254915/676ece0cb3b8/jvirol00118-0209-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1867/254915/15d12fae2bd9/jvirol00118-0210-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1867/254915/c312298366ba/jvirol00118-0211-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1867/254915/466379eb11ea/jvirol00118-0212-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1867/254915/eabb11ff1d9d/jvirol00118-0213-a.jpg

相似文献

1
Transformation of Brown Leghorn chicken embryo fibroblasts by avian myeloblastosis virus proviral DNA.禽成髓细胞瘤病毒前病毒DNA对来航鸡胚成纤维细胞的转化
J Virol. 1985 Jul;55(1):193-205. doi: 10.1128/JVI.55.1.193-205.1985.
2
Transforming potential of the v-myb oncogene from avian myeloblastosis virus: alterations in the oncogene product may reveal a new target specificity.禽成髓细胞瘤病毒v-myb癌基因的转化潜能：癌基因产物的改变可能揭示一种新的靶标特异性。
Biochimie. 1986 Jul-Aug;68(7-8):969-80. doi: 10.1016/s0300-9084(86)80040-2.
3
Acute leukemia viruses E26 and avian myeloblastosis virus have related transformation-specific RNA sequences but different genetic structures, gene products, and oncogenic properties.急性白血病病毒E26和禽成髓细胞瘤病毒具有相关的转化特异性RNA序列，但遗传结构、基因产物和致癌特性不同。
Proc Natl Acad Sci U S A. 1982 Jun;79(12):3677-81. doi: 10.1073/pnas.79.12.3677.
4
Genetic structure of avian myeloblastosis virus, released from transformed myeloblasts as a defective virus particle.禽成髓细胞瘤病毒的遗传结构，作为一种缺陷病毒粒子从转化的成髓细胞中释放出来。
Proc Natl Acad Sci U S A. 1980 Sep;77(9):5120-4. doi: 10.1073/pnas.77.9.5120.
5
Expression of a truncated v-myb product in transformed chicken embryo fibroblasts.截短的v-myb产物在转化的鸡胚成纤维细胞中的表达。
FEBS Lett. 1987 Apr 6;214(1):81-6. doi: 10.1016/0014-5793(87)80017-0.
6
Cellular sequences are present in the presumptive avian myeloblastosis virus genome.细胞序列存在于假定的禽成髓细胞瘤病毒基因组中。
Proc Natl Acad Sci U S A. 1980 Sep;77(9):5177-81. doi: 10.1073/pnas.77.9.5177.
7
Identification of the avian myeloblastosis virus genome. II. Restriction endonuclease analysis of DNA from lambda proviral recombinants and leukemic myeoblast clones.禽成髓细胞瘤病毒基因组的鉴定。II. λ 原病毒重组体和白血病成髓细胞克隆的 DNA 的限制性内切酶分析。
J Virol. 1980 Nov;36(2):325-36. doi: 10.1128/JVI.36.2.325-336.1980.
8
Expression of molecular clones of v-myb in avian and mammalian cells independently of transformation.v-myb分子克隆在禽细胞和哺乳动物细胞中的表达，与转化无关。
J Virol. 1986 Aug;59(2):267-75. doi: 10.1128/JVI.59.2.267-275.1986.
9
Size and genetic content of virus-specific RNA in myeloblasts transformed by avian myeloblastosis virus (AMV).禽成髓细胞瘤病毒（AMV）转化的成髓细胞中病毒特异性RNA的大小和遗传内容。
Virology. 1981 Apr 15;110(1):128-36. doi: 10.1016/0042-6822(81)90014-3.
10
Effects of 5' and 3' truncations of the myb gene on the transforming ability of avian myeloblastosis virus (AMV).myb基因5'端和3'端截短对禽成髓细胞瘤病毒（AMV）转化能力的影响。
Virology. 1990 Apr;175(2):562-7. doi: 10.1016/0042-6822(90)90441-s.

引用本文的文献

1
Alterations of the MDV oncogenic regions in an MDV transformed lymphoblastoid cell line.马立克氏病病毒转化的淋巴母细胞样细胞系中马立克氏病病毒致癌区域的改变。
Mol Pathol. 2002 Aug;55(4):262-72. doi: 10.1136/mp.55.4.262.
2
Retroviral insertional activation of the c-myb proto-oncogene in a Marek's disease T-lymphoma cell line.马立克氏病T淋巴瘤细胞系中c-myb原癌基因的逆转录病毒插入激活
J Virol. 1996 Nov;70(11):7414-23. doi: 10.1128/JVI.70.11.7414-7423.1996.
3
Overexpression of C-terminally but not N-terminally truncated Myb induces fibrosarcomas: a novel nonhematopoietic target cell for the myb oncogene.

本文引用的文献

1
Transcripts from the cellular homologs of retroviral oncogenes: distribution among chicken tissues.逆转录病毒致癌基因细胞同源物的转录本：在鸡组织中的分布
Mol Cell Biol. 1982 Jun;2(6):617-24. doi: 10.1128/mcb.2.6.617-624.1982.
2
AGAR SUSPENSION CULTURE FOR THE SELECTIVE ASSAY OF CELLS TRANSFORMED BY POLYOMA VIRUS.用于多瘤病毒转化细胞选择性测定的琼脂悬浮培养
Virology. 1964 Jun;23:291-4. doi: 10.1016/0042-6822(64)90301-0.
3
AVIAN VIRUS GROWTHS AND THEIR ETIOLOGIC AGENTS.禽病毒生长及其病原体
C末端而非N末端截短的Myb过表达会诱导纤维肉瘤形成：一种myb癌基因的新型非造血靶细胞。
Mol Cell Biol. 1994 Apr;14(4):2278-90. doi: 10.1128/mcb.14.4.2278-2290.1994.
4
Stimulation of an alpha like DNA polymerase by v-myc related protein of Halobacterium halobium.嗜盐栖嗜盐菌的v-myc相关蛋白对α类DNA聚合酶的刺激作用。
Arch Microbiol. 1988 Jan;149(3):175-80. doi: 10.1007/BF00422002.
5
Induction of nephroblastoma by myeloblastosis-associated virus type 1: state of proviral DNAs in tumor cells.1型成髓细胞增多症相关病毒诱导肾母细胞瘤：肿瘤细胞中前病毒DNA的状态
J Virol. 1989 Apr;63(4):1803-7. doi: 10.1128/JVI.63.4.1803-1807.1989.
6
Proviral rearrangements and overexpression of a new cellular gene (nov) in myeloblastosis-associated virus type 1-induced nephroblastomas.原病毒重排及新细胞基因（nov）在1型成髓细胞瘤相关病毒诱导的肾母细胞瘤中的过表达。
Mol Cell Biol. 1992 Jan;12(1):10-21. doi: 10.1128/mcb.12.1.10-21.1992.
Adv Cancer Res. 1963;7:1-127. doi: 10.1016/s0065-230x(08)60982-3.
4
Properties of cells infected with avian myeloblastosis virus.感染禽成髓细胞瘤病毒的细胞特性。
Cold Spring Harb Symp Quant Biol. 1962;27:415-25. doi: 10.1101/sqb.1962.027.001.039.
5
Morphological conversion of cell cultures by avian myeloblastosis virus.禽成髓细胞瘤病毒对细胞培养物的形态学转化
Virology. 1961 Oct;15:185-99. doi: 10.1016/0042-6822(61)90234-3.
6
Virus of avian myeloblastosis. XIV. Neoplastic response of normal chicken bone marrow treated with the virus in tissue culture.禽成髓细胞瘤病毒。十四。在组织培养中用该病毒处理的正常鸡骨髓的肿瘤反应。
J Natl Cancer Inst. 1960 Feb;24:395-415.
7
Proposal for naming host cell-derived inserts in retrovirus genomes.关于逆转录病毒基因组中宿主细胞衍生插入片段命名的提议。
J Virol. 1981 Dec;40(3):953-7. doi: 10.1128/JVI.40.3.953-957.1981.
8
Target cells for avian myeloblastosis virus in embryonic yolk sac and relationship of cell differentiation to cell transformation.胚胎卵黄囊中禽成髓细胞瘤病毒的靶细胞以及细胞分化与细胞转化的关系。
J Virol. 1984 Mar;49(3):841-7. doi: 10.1128/JVI.49.3.841-847.1984.
9
Subcellular localization of proteins encoded by oncogenes of avian myeloblastosis virus and avian leukemia virus E26 and by chicken c-myb gene.禽成髓细胞瘤病毒和禽白血病病毒E26的癌基因以及鸡c-myb基因所编码蛋白质的亚细胞定位。
Cell. 1984 Jun;37(2):537-47. doi: 10.1016/0092-8674(84)90384-2.
10
A putative second cell-derived oncogene of the avian leukaemia retrovirus E26.禽白血病逆转录病毒E26一种假定的第二种细胞衍生癌基因。
Nature. 1983;306(5941):395-7. doi: 10.1038/306395a0.