Klempnauer K H, Symonds G, Evan G I, Bishop J M
Cell. 1984 Jun;37(2):537-47. doi: 10.1016/0092-8674(84)90384-2.
Analysis of the subcellular location of the proteins encoded by the oncogenes of avian myeloblastosis virus and avian leukemia virus E26 ( p45v -myb and p135gag -myb-ets, respectively) and by the chicken c-myb gene ( p75c -myb) shows that all three proteins are located in the nucleus. In AMV-infected (but not transformed) chicken fibroblasts p45v -myb also resides in the nucleus, indicating that a nuclear location of p45v -myb in these cells is not sufficient to achieve transformation. In AMV-transformed myeloblasts a small fraction of p45v -myb occupies an additional site in the perinuclear region of the cytoplasm. If the myeloblasts are caused to differentiate to macrophages, most of p45v -myb is found in the cytoplasm. This redistribution of p45v -myb within the cell may be responsible for reversion of the transformed phenotype.
对禽成髓细胞瘤病毒和禽白血病病毒E26（分别为p45v -myb和p135gag -myb-ets）的癌基因以及鸡c-myb基因（p75c -myb）所编码蛋白质的亚细胞定位分析表明，这三种蛋白质均位于细胞核中。在感染了禽成髓细胞瘤病毒（但未发生转化）的鸡成纤维细胞中，p45v -myb也存在于细胞核中，这表明在这些细胞中p45v -myb的核定位不足以实现转化。在禽成髓细胞瘤病毒转化的成髓细胞中，一小部分p45v -myb在细胞质的核周区域占据了一个额外的位点。如果使成髓细胞分化为巨噬细胞，则大部分p45v -myb存在于细胞质中。p45v -myb在细胞内的这种重新分布可能是转化表型逆转的原因。
