用于检查……中晶状体再生的方法
Methods for Examining Lens Regeneration in .
作者信息
Henry Jonathan J, Perry Kimberly J, Hamilton Paul W
机构信息
Department of Cell and Developmental Biology, University of Illinois, Urbana, Illinois 61801;
Department of Cell and Developmental Biology, University of Illinois, Urbana, Illinois 61801.
出版信息
Cold Spring Harb Protoc. 2019 Apr 1;2019(4):pdb.prot101527. doi: 10.1101/pdb.prot101527.
Some vertebrates are able to regenerate the lens following its removal. This includes species in the genus (i.e., , , and ), the only anurans known to undergo lens regeneration. In the regenerated lens is derived de novo from cells located within the basal-most layer of the larval corneal epithelium, and is triggered by factors provided by the neural retina. In larval frogs the corneal epithelium is underlain by an endothelium separated from the corneal epithelium except for a small central attachment (i.e., the "stromal-attracting center"). This connection grows larger as the stroma forms and the frogs approach metamorphosis. Here we provide instructions for performing lentectomies (removal of the original lens) to study lens regeneration.
一些脊椎动物在晶状体被摘除后能够再生晶状体。这包括 属的物种（即 、 和 ），它们是已知的仅有的能进行晶状体再生的无尾两栖类动物。在 中，再生晶状体从头起源于位于幼虫角膜上皮最底层的细胞，并由神经视网膜提供的因子触发。在幼虫青蛙中，角膜上皮下方是一层内皮，除了一个小的中央附着点（即“基质吸引中心”）外，内皮与角膜上皮分离。随着基质形成和青蛙接近变态，这种连接会变大。在这里，我们提供了进行晶状体摘除术（摘除原始晶状体）以研究晶状体再生的操作指南。
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