Reeve J G, Wild A E
J Embryol Exp Morphol. 1978 Dec;48:205-14.
In Xenopus laevis tadpoles, wounding of the outer cornea failed to initiate lens regeneration. If both the outer and inner corneas were wounded or if the lens was dislocated, lens regeneration was initiated but failed to continue beyond stage III. However, lensectomy followed by re-implantation of the lens resulted in the regeneration of a fully differentiated lens in several cases, despite the presence of the re-implanted lens. Although some of the regenerates in these eyes were also arrested at stage III, those which attained full lens differentiation, i.e. stage V, developed normally and synthesized crystalline from the onset of stage IV as indicated by a positive immunofluorescence reaction. Histological examination of the dislocated and re-implanted lenses showed the majority of them to be normal in appearance. Cornea transplanted to the posterior chamber of the eye also regenerated a lens in the presence of the re-implanted lens. All these regenerates underwent lens fibre differentiation to give stage-V regenerates. These findings show that lens regeneration from the cornea can occur in the presence of lens. Results are discussed on the basis that contrary to earlier suggestions, an inhibitory lens factor does not exist in vivo, but rather that a factor for the initiation and maintenance of regeneration emanates from the eye cup and upon wounding of the inner cornea is able to reach the inner cell layer of the outer cornea and initiate lens regeneration.
在非洲爪蟾蝌蚪中，外角膜受伤无法启动晶状体再生。如果外角膜和内角膜都受伤，或者晶状体脱位，晶状体再生会启动，但在III期之后无法继续。然而，晶状体摘除后再植入晶状体，在一些情况下会导致完全分化的晶状体再生，尽管存在再植入的晶状体。虽然这些眼睛中的一些再生晶状体也在III期停滞，但那些达到完全晶状体分化（即V期）的晶状体，从IV期开始就正常发育并合成晶状体蛋白，免疫荧光反应呈阳性表明了这一点。对脱位并再植入的晶状体进行组织学检查，发现大多数外观正常。移植到眼后房的角膜在存在再植入晶状体的情况下也能再生晶状体。所有这些再生晶状体都经历了晶状体纤维分化，形成了V期再生晶状体。这些发现表明，在存在晶状体的情况下，角膜也能再生晶状体。基于以下观点对结果进行了讨论：与早期观点相反，体内不存在抑制性晶状体因子，而是启动和维持再生的因子来自眼杯，在内角膜受伤时能够到达外角膜的内细胞层并启动晶状体再生。
