Roberts C R, Weir A C, Hay J, Straus S E, Ruyechan W T
J Virol. 1985 Jul;55(1):45-53. doi: 10.1128/JVI.55.1.45-53.1985.
DNA-binding proteins present in varicella-zoster virus-infected cells were identified by DNA-cellulose chromatography of radioactively labeled cell extracts. Seven virus-specific proteins, ranging in molecular weight from approximately 175,000 to 21,000, showed affinity for single- or double-stranded DNA or both. These proteins include the varicella-zoster virus major capsid protein, a phosphorylated tegument protein, and a 125,000-molecular-weight species which may be analogous to the major DNA-binding protein of herpes simplex virus. We also identified a number of DNA-binding phosphoproteins by these procedures. Finally, protein blot studies were carried out to determine whether these proteins bind preferentially to virus rather than to host cell DNA.
通过对放射性标记的细胞提取物进行DNA纤维素层析，鉴定了水痘带状疱疹病毒感染细胞中存在的DNA结合蛋白。七种病毒特异性蛋白，分子量约为175,000至21,000，对单链或双链DNA或两者都显示出亲和力。这些蛋白质包括水痘带状疱疹病毒主要衣壳蛋白、一种磷酸化的被膜蛋白，以及一种分子量为125,000的蛋白，它可能类似于单纯疱疹病毒的主要DNA结合蛋白。我们还通过这些方法鉴定了一些DNA结合磷蛋白。最后，进行了蛋白质印迹研究，以确定这些蛋白质是否优先结合病毒而非宿主细胞DNA。
