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一种针对猴病毒40控制区晚期编码链的序列特异性单链结合蛋白。

A sequence-specific single-strand-binding protein for the late-coding strand of the simian virus 40 control region.

作者信息

Gaillard C, Weber M, Strauss F

机构信息

Institut Jacques Monod, Paris, France.

出版信息

J Virol. 1988 Jul;62(7):2380-5. doi: 10.1128/JVI.62.7.2380-2385.1988.

DOI:10.1128/JVI.62.7.2380-2385.1988
PMID:2836621
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC253395/
Abstract

We have purified a protein from uninfected monkey CV1 cells that binds specifically in vitro to the late-coding simian virus 40 DNA strand in the region of transcription control without any detectable binding to the complementary single strand. Nuclease protection experiments detected two binding sites in the 21-base-pair repeat region. The protein did not bind to this region in the double-stranded form, nor did it bind to RNA synthesized in vitro by using either DNA strand as a template. This protein, and perhaps other DNA single-strand-sequence-specific proteins, may play a role in the control of gene expression in higher organisms.

摘要

我们已从未感染的猴CV1细胞中纯化出一种蛋白质，该蛋白质在体外可特异性结合转录控制区域中猿猴病毒40晚期编码DNA链，而与互补单链无任何可检测到的结合。核酸酶保护实验在21个碱基对的重复区域检测到两个结合位点。该蛋白质不以双链形式结合此区域，也不与以任何一条DNA链为模板体外合成的RNA结合。这种蛋白质，或许还有其他DNA单链序列特异性蛋白质，可能在高等生物的基因表达调控中发挥作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3d1a/253395/a771f72c7c8f/jvirol00086-0184-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3d1a/253395/3ccee15c84c3/jvirol00086-0181-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3d1a/253395/d07269e114e9/jvirol00086-0182-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3d1a/253395/0ba6ce5192ed/jvirol00086-0183-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3d1a/253395/a771f72c7c8f/jvirol00086-0184-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3d1a/253395/3ccee15c84c3/jvirol00086-0181-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3d1a/253395/d07269e114e9/jvirol00086-0182-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3d1a/253395/0ba6ce5192ed/jvirol00086-0183-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3d1a/253395/a771f72c7c8f/jvirol00086-0184-a.jpg

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本文引用的文献

1
The binding of a transcription factor to deletion mutants of a 5S ribosomal RNA gene.转录因子与5S核糖体RNA基因缺失突变体的结合。
Cell. 1981 Mar;23(3):665-9. doi: 10.1016/0092-8674(81)90429-3.
2
A specific transcription factor that can bind either the 5S RNA gene or 5S RNA.一种能与5S RNA基因或5S RNA结合的特异性转录因子。
Proc Natl Acad Sci U S A. 1980 Jul;77(7):4170-4. doi: 10.1073/pnas.77.7.4170.
3
A protein binds to a satellite DNA repeat at three specific sites that would be brought into mutual proximity by DNA folding in the nucleosome.
以线性聚丙烯酰胺为载体进行DNA的乙醇沉淀。
Nucleic Acids Res. 1990 Jan 25;18(2):378. doi: 10.1093/nar/18.2.378.
4
Programmed factor binding to simian virus 40 GC-box replication and transcription control sequences.程序化因子与猿猴病毒40 GC盒复制及转录控制序列的结合。
J Virol. 1990 Jan;64(1):347-53. doi: 10.1128/JVI.64.1.347-353.1990.
5
A single-stranded DNA binding protein from S. cerevisiae specifically recognizes the T-rich strand of the core sequence of ARS elements and discriminates against mutant sequences.来自酿酒酵母的一种单链DNA结合蛋白能特异性识别自主复制序列（ARS）元件核心序列中富含胸腺嘧啶（T）的链，并区分突变序列。
EMBO J. 1991 Apr;10(4):981-5. doi: 10.1002/j.1460-2075.1991.tb08032.x.
6
Single strand binding protein specific for the polyoma early-coding strand of PEA1 (AP1) regulatory sequence.对多瘤病毒早期编码链PEA1（AP1）调控序列具有特异性的单链结合蛋白。
Nucleic Acids Res. 1991 Aug 11;19(15):4279-87. doi: 10.1093/nar/19.15.4279.
7
Use of gel retardation to analyze protein-nucleic acid interactions.使用凝胶阻滞分析法分析蛋白质-核酸相互作用。
Microbiol Rev. 1992 Dec;56(4):509-28. doi: 10.1128/mr.56.4.509-528.1992.
8
Sequence of cDNA comprising the human pur gene and sequence-specific single-stranded-DNA-binding properties of the encoded protein.包含人类嘌呤基因的cDNA序列以及所编码蛋白质的序列特异性单链DNA结合特性。
Mol Cell Biol. 1992 Dec;12(12):5673-82. doi: 10.1128/mcb.12.12.5673-5682.1992.
9
A nuclear single-stranded-DNA binding factor interacts with the long terminal repeats of the 1731 Drosophila retrotransposon.
J Virol. 1992 Mar;66(3):1682-7. doi: 10.1128/JVI.66.3.1682-1687.1992.
10
Two synthetic Sp1-binding sites functionally substitute for the 21-base-pair repeat region to activate simian virus 40 growth in CV-1 cells.两个合成的Sp1结合位点在功能上替代了21个碱基对的重复区域，以激活猿猴病毒40在CV-1细胞中的生长。
J Virol. 1992 Nov;66(11):6379-90. doi: 10.1128/JVI.66.11.6379-6390.1992.
一种蛋白质在三个特定位点与卫星DNA重复序列结合，这些位点在核小体中会通过DNA折叠而相互靠近。
Cell. 1984 Jul;37(3):889-901. doi: 10.1016/0092-8674(84)90424-0.
4
Binding of Xenopus transcription factor A to 5S RNA and to single stranded DNA.非洲爪蟾转录因子A与5S RNA及单链DNA的结合。
Nucleic Acids Res. 1984 Mar 26;12(6):2745-58. doi: 10.1093/nar/12.6.2745.
5
Specific interaction between enhancer-containing molecules and cellular components.含增强子分子与细胞成分之间的特异性相互作用。
Cell. 1984 Feb;36(2):403-11. doi: 10.1016/0092-8674(84)90233-2.
6
The promoter-specific transcription factor Sp1 binds to upstream sequences in the SV40 early promoter.启动子特异性转录因子Sp1与SV40早期启动子中的上游序列结合。
Cell. 1983 Nov;35(1):79-87. doi: 10.1016/0092-8674(83)90210-6.
7
Equilibria and kinetics of lac repressor-operator interactions by polyacrylamide gel electrophoresis.通过聚丙烯酰胺凝胶电泳研究乳糖阻遏物-操纵基因相互作用的平衡与动力学
Nucleic Acids Res. 1981 Dec 11;9(23):6505-25. doi: 10.1093/nar/9.23.6505.
8
Studies on the structure of mouse helix-destabilizing protein-1. DNA binding and controlled proteolysis with trypsin.小鼠解螺旋蛋白-1结构的研究。与胰蛋白酶的DNA结合及可控蛋白水解作用。
J Biol Chem. 1980 Dec 10;255(23):11547-56.
9
Isolation of transcription factors that discriminate between different promoters recognized by RNA polymerase II.区分RNA聚合酶II识别的不同启动子的转录因子的分离。
Cell. 1983 Mar;32(3):669-80. doi: 10.1016/0092-8674(83)90053-3.
10
Specific interaction of a purified transcription factor with an internal control region of 5S RNA genes.一种纯化的转录因子与5S RNA基因内部调控区的特异性相互作用。
Cell. 1980 Mar;19(3):717-28. doi: 10.1016/s0092-8674(80)80048-1.