Radhakrishna Auji, Dwivedi Krishna Kumar, Srivastava Manoj Kumar, Roy A K, Malaviya D R, Kaushal P
ICAR-Indian Grassland and Fodder Research Institute, Jhansi 284003, UP, India.
ICAR-Indian Institute of Sugarcane Research, Lucknow 226002, UP, India.
Data Brief. 2018 Mar 10;18:590-593. doi: 10.1016/j.dib.2018.03.001. eCollection 2018 Jun.
Guinea grass ( Jacq), an important fodder crop of humid and sub-humid tropical regions, reproduces through apomixis, a method of clonal propagation through seeds. Lack of knowledge of the genetic and molecular control of this phenomena has hindered the genetic improvement of this crop. The dataset provided here represents the first RNA-Seq based assembly and analysis of florets at pre-meiotic stage from the apomictic and sexual genotypes of guinea grass. The raw sequence files in FASTQ format were deposited in the NCBI SRA database with accession number SRP115883. A total of 24.8 Gb raw sequence data, corresponding to 17,96,65,827 raw reads was obtained by paired end sequencing. We used Trinity for de-novo assembly and identified 57,647 transcripts in sexual and 49,093 transcripts in apomictic type. This transcriptome data will be useful for identification and comparative analysis of genes regulating the mode of reproduction in grasses.
臂形草（Panicum maximum Jacq）是湿润和亚湿润热带地区一种重要的饲料作物，通过无融合生殖进行繁殖，这是一种通过种子进行克隆繁殖的方法。对这种现象的遗传和分子控制缺乏了解阻碍了该作物的遗传改良。这里提供的数据集代表了基于RNA测序对臂形草无融合生殖和有性生殖基因型减数分裂前期小花的首次组装和分析。FASTQ格式的原始序列文件已存入NCBI SRA数据库，登录号为SRP115883。通过双末端测序获得了总共24.8 Gb的原始序列数据，对应179665827条原始读段。我们使用Trinity进行从头组装，在有性生殖类型中鉴定出57647个转录本，在无融合生殖类型中鉴定出49093个转录本。该转录组数据将有助于鉴定和比较分析调控禾本科植物繁殖方式的基因。
