通过检测L型钙通道蛋白及其相关蛋白的表达，比较针刺内关穴、列缺穴及非穴位对心肌缺血（MI）大鼠心肌细胞功能的有益影响。
Comparison of Beneficial Effects of Acupuncture at PC6, LU7 & Non- acupuncture Points on Cardiac Cell Function in Myocardial Ischemia (MI) Rats by Testing Protein Expression of L-type Calcium Channel Protein and its Related Protein.
作者信息
Liu Longtao, Wang Songzi, Fu Chnnoceno, Shu Shi, Fu Martin, Li Jing, Deng Xue, Sun Jianduan, Chen Wenna, Ren Lu
出版信息
Acupunct Electrother Res. 2016;41(2):107-125. doi: 10.3727/036012916x14666839504631.
The mechanism of why electro-acupuncture (EA) at PC6 improves the heart function was investigated by studying how the L-type cardiac voltage-dependent calcium channel in myocardial ischemia (MI) is regulated. Cava,., Cavo and Cava2-61 are main component proteins of L-type calcium channel; CaM and Calmodulin kinase II (CaMKII) are Ca2+ channel associated proteins. In this experiment, MI was induced by injection of isoproterenol (ISO) in rats and electrocardiograms (ECGs) were recorded before and after every injection, and protein expressions of Cava,c, Cavp, Cava2_61, CaM and CaMKII were higher [The protein expression increased 43.39%, 54.85%, 47.08%, 48.29% and 50.36% respectively] than the control rats significantly (p<0.05). After MI induction, the MI rats were divided into three groups, including PC6 (Neiguan-point), LU7 (Lieque-point) and Non-acupuncture-point group, which were acupunctured once a day for 7 days respectively. After EA at PC6, the protein expressions showed obvious decrease [EA at PC6: the protein expressions of Cava1c, CavP, Cava2-81, CaM and CaMKII decreased 26.36%, 27.58%, 25.21%, 27.21% and 26.61% respectively.] and they are all lower than MI rats significantly (p<0.05). After EA at LU7 and Non-acupuncture-point, the protein expressions showed no significant changes. The effect of EA at PC6 was significantly better than LU7 and Non- acupuncture-point (p<0.05). PC6 is an acupoint of the pericardium meridian, and the pericardium meridian, which corresponds to opioid system according to Li P's research, can affect the cardio-vascular function directly. LU7 is located on the lung meridian; it cannot affect the heart function directly although the lung is related to the heart in blood circulation function so PC6 showed the target treating effect of meridian specificity on regulating the L-type calcium channel in MI.
通过研究心肌缺血（MI）时L型心脏电压依赖性钙通道如何被调节，探讨了针刺内关穴改善心脏功能的机制。Cavα1c、Cavβ、Cavα2-δ1是L型钙通道的主要组成蛋白；钙调蛋白（CaM）和钙调蛋白激酶II（CaMKII）是与Ca2+通道相关的蛋白。本实验通过给大鼠注射异丙肾上腺素（ISO）诱导MI，并在每次注射前后记录心电图（ECG），结果显示，MI大鼠的Cavα1c、Cavβ、Cavα2-δ1、CaM和CaMKII蛋白表达[蛋白表达分别增加43.39%、54.85%、47.08%、48.29%和50.36%]显著高于对照组大鼠（p<0.05）。诱导MI后，将MI大鼠分为三组，分别为针刺内关穴组、针刺列缺穴组和非针刺穴位组，每组每天针刺1次，连续7天。针刺内关穴后，蛋白表达明显降低[针刺内关穴：Cavα1c、Cavβ、Cavα2-δ1、CaM和CaMKII蛋白表达分别降低26.36%、27.58%、25.21%、27.21%和26.61%]，且均显著低于MI大鼠（p<0.05）。针刺列缺穴和非针刺穴位后，蛋白表达无明显变化。针刺内关穴的效果显著优于针刺列缺穴和非针刺穴位（p<0.05）。内关穴是心包经的穴位，根据李平的研究，心包经与阿片系统相对应，可直接影响心血管功能。列缺穴位于肺经上；虽然肺在血液循环功能上与心脏相关，但它不能直接影响心脏功能，因此内关穴在调节MI时的L型钙通道方面显示出经络特异性的靶向治疗作用。
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