Zhao Wan-Shuang, Li Di, Xu Ya-Han, Li Meng-di, Chen Jin, Kou Zhi-Jun, Wang Qi-Ge, Chen Yi-Guo, Joseph Nsoa Dimitri
Dept. of Liaoning University of Traditional Chinese Medicine, Shenyang 110847, China.
Dept. of Liaoning University of Traditional Chinese Medicine, Shenyang 110847, China.
J Acupunct Meridian Stud. 2018 Jun;11(3):88-96. doi: 10.1016/j.jams.2018.03.002. Epub 2018 Mar 31.
This study aims to investigate the possible mechanisms of electroacupuncture (EA) at PC6 to improve myocardial ischemia (MI) by regulating the cardiac transient outward potassium current channel (Ito). According to the random number table, the mice were divided into six groups of six mice each: control group, MI group, PC6, LU7 (Lieque-point), ST36 (Zusanli-point), and nonacupoint group. Mice in the control group were injected with saline (20 mg/kg, 24 hours interval), and the other ASIC3 -/- mice were injected subcutaneously twice with isoproterenol (ISO) (20 mg/kg, 24 hours interval). In the preexperiment, 5 mg/kg, 10 mg/kg, 20 mg/kg, and 30 mg/kg of ISO were used, and the results showed that 5 mg/kg and 10 mg/kg of ISO both could induce acute MI, but shorter duration of sustained MI. On the other hand, an injection of 30 mg/kg can make the mice experience arrhythmia or die immediately, and EA was operated at PC6, LU7, ST36 acupoints, and nonacupoint in the mice of PC6, LU7, ST36, and nonacupoint groups, respectively, after injecting twice. Then Western blotting techniques (Western Blot) were used to analyze the protein expressions of Kv1.4, Kv4.2, Kv4.3, and KchIP2. The results of this experiment showed that the protein expressions of Kv1.4, Kv4.2, Kv4.3, and KChIP2 in MI group were significantly lower than those in the control group (p < 0.01). Compared with MI group, the results of PC6, LU7, and ST36 groups obviously increased (p < 0.05). Furthermore, the expressions of PC6 group were higher than LU7 group and ST36 group (p < 0.05). And electrocardiogram's T-waves showed obvious pathological changes in the MI group compared to the control group (p < 0.01). After EA, the abnormal T-waves voltage of ECG in PC6, LU7, and ST36 groups was improved (p < 0.05). In addition, the rate change of PC6 group was larger than that of both LU7 and ST36 groups (p < 0.05). But the T-waves voltage of the nonacupoint group was not significantly different than that of the MI group (p > 0.05).
本研究旨在探讨针刺内关穴改善心肌缺血（MI）的可能机制，即通过调节心脏瞬时外向钾电流通道（Ito）来实现。根据随机数字表，将小鼠分为六组，每组六只：对照组、MI组、内关穴组、列缺穴组（LU7）、足三里穴组（ST36）和非穴位组。对照组小鼠注射生理盐水（20mg/kg，间隔24小时），其他ASIC3 -/-小鼠皮下注射异丙肾上腺素（ISO）两次（20mg/kg，间隔24小时）。在预实验中，使用了5mg/kg、10mg/kg、20mg/kg和30mg/kg的ISO，结果显示5mg/kg和10mg/kg的ISO均可诱导急性MI，但持续性MI的持续时间较短。另一方面，注射30mg/kg可使小鼠出现心律失常或立即死亡，在分别注射两次后，对PC6、LU7、ST36组和非穴位组的小鼠分别在内关穴、列缺穴、足三里穴和非穴位处进行电针操作。然后采用蛋白质印迹技术（Western Blot）分析Kv1.4、Kv4.2、Kv4.3和KchIP2的蛋白表达。本实验结果显示，MI组中Kv1.4、Kv4.2、Kv4.3和KChIP2的蛋白表达明显低于对照组（p < 0.01）。与MI组相比，PC6、LU7和ST36组的结果明显升高（p < 0.05）。此外，PC6组的表达高于LU7组和ST36组（p < 0.05）。与对照组相比，MI组心电图的T波显示出明显的病理变化（p < 0.01）。电针后，PC6、LU7和ST36组心电图异常T波电压得到改善（p < 0.05）。此外，PC6组的变化率大于LU7组和ST36组（p < 0.05）。但非穴位组的T波电压与MI组无显著差异（p > 0.05）。
