Wang Ying, Zhang Xiao-Lu, Wang Wei, Li Di, Dai Jian-Yu, Li Chun-Ri, Li Ji-Quan, Chen Yi-Guo, Rong Pei-Jing
Acupunct Electrother Res. 2015;40(4):335-53. doi: 10.3727/036012916x14533115160642.
This study is aim to investigate the effect of electro-acupuncture at PC6 (Neiguan-point) on the gene and protein expressions of classical inward-rectifier potassium channels (Kir) in myocardial ischemia (MI) rats induced by isoproterenol (ISO). With ten for each one, 50 rats were divided into 5 groups which were control group, MI group, PC6 group, LU7 (Lieque-point) group and non-acupoint group. The control group was injected normal saline solution (85 mg/kg), the other groups were injected ISO (85 mg/kg). All the rats were injected once daily for two days and recorded electrocardiograms (ECGs) after every injection. Electro-acupuncture (EA) was operated at PC6, LU7 and non-acupoint respectively in the rats of PC6 group, LU7 group and non-acupoint group after twice injections. EA was performed to these three groups with disperse-dense wave (4-20 Hz), pulse amplitude of 14V, 20 mins a day remaining 7 days. The gene and protein expressions of Kir2.1, Kir2.2 and Kir2.3 were analyzed by Western Immunoblotting Technology (Western Blot) and Real-time Fluorescence Quantitative Polymerase Chain Reaction (RT-PCR). But it is regrettable that we did not detect meaningful gene and protein expressions Kir2.3, and the expressions of Kir2.1 and Kir2.2 in MI induced groups were lower [The gene and protein decreased 39.4 ± 27.3% and 38.7 ± 17.1% respectively.] than control group (P < 0.05). Compared with MI group, the results of PC6 group and LU7 group increased [PC6 group: the gene and protein increased 42.9 25.0% and 42.2 ± 10.0% respectively. LU7 group: the gene and protein increased 23.8 ± 50.1% and 21.1 ± 32.5% respectively.] obviously (P < 0.05) after EA, furthermore the expressions of PC6 group were higher [The gene and protein increased 15.4 ± 16.7% and 17.3 ± 60% respectively.] than LU7 group (P < 0.05). The results show that PC6 has a better positive effect than LU7 on MI rats, and the mechanism is probably that EA at PC6 can significantly increase the gene and protein expressions of Kir2.1 and Kir2.2.
本研究旨在探讨电针内关穴对异丙肾上腺素（ISO）诱导的心肌缺血（MI）大鼠经典内向整流钾通道（Kir）基因和蛋白表达的影响。将50只大鼠，每组10只，分为5组，即对照组、MI组、内关穴组、列缺穴组和非穴位组。对照组注射生理盐水（85mg/kg），其他组注射ISO（85mg/kg）。所有大鼠每天注射1次，连续注射2天，每次注射后记录心电图（ECG）。在两次注射后，分别对内关穴组、列缺穴组和非穴位组的大鼠进行内关穴、列缺穴和非穴位的电针操作。对这三组进行疏密波（4-20Hz）电针，脉冲幅度为14V，每天20分钟，持续7天。采用蛋白质免疫印迹技术（Western Blot）和实时荧光定量聚合酶链反应（RT-PCR）分析Kir2.1、Kir2.2和Kir2.3的基因和蛋白表达。但遗憾的是，我们未检测到有意义的Kir2.3基因和蛋白表达，且MI诱导组中Kir2.1和Kir2.2的表达低于对照组[基因和蛋白分别下降39.4±27.3%和38.7±17.1%]（P<0.05）。与MI组相比，电针后内关穴组和列缺穴组的结果明显升高[内关穴组：基因和蛋白分别升高42.9±25.0%和42.2±10.0%。列缺穴组：基因和蛋白分别升高23.8±50.1%和21.1±32.5%]（P<0.05）；此外，内关穴组的表达高于列缺穴组[基因和蛋白分别升高15.4±16.7%和17.3±60%]（P<0.05）。结果表明，内关穴对MI大鼠的正向作用优于列缺穴，其机制可能是电针内关穴可显著增加Kir2.1和Kir2.2的基因和蛋白表达。
