小鼠精子主要卫星DNA的低甲基化模式

Pattern of undermethylation of the major satellite DNA of mouse sperm.

作者信息

Feinstein S I, Racaniello V R, Ehrlich M, Gehrke C W, Miller D A, Miller O J

出版信息

Nucleic Acids Res. 1985 Jun 11;13(11):3969-78. doi: 10.1093/nar/13.11.3969.

DOI:10.1093/nar/13.11.3969

PMID:2989780

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC341290/

Abstract

Enzymatic hydrolysis and base analysis by high performance liquid chromatography showed that mouse satellite DNA had 30-50% less 5-methylcytosine in sperm than in somatic tissue (1.59 mols % vs 2.40-3.11 mols %). Maxam-Gilbert sequencing and analysis of the intensity of the cytosine bands indicated that the level of methylation of the eight CpGs of the consensus sequence in sperm satellite DNA ranged from 0 to about 50%, considerably lower than the levels reported in somatic tissues. The Mn1I site containing one of these CpGs was cut much more extensively in satellite DNA from sperm than from liver, confirming the undermethylation of this site in sperm DNA.

摘要

酶促水解及高效液相色谱法碱基分析表明，小鼠卫星DNA在精子中的5-甲基胞嘧啶比在体细胞组织中少30%-50%（1.59摩尔%对2.40-3.11摩尔%）。Maxam-Gilbert测序及胞嘧啶条带强度分析表明，精子卫星DNA共有序列中8个CpG的甲基化水平在0至约50%之间，显著低于体细胞组织中报道的水平。包含这些CpG之一的Mn1I位点在精子卫星DNA中比在肝脏卫星DNA中被切割得更广泛，证实了该位点在精子DNA中的甲基化不足。