Mazzi-Chaves Jardel Francisco, Petean Igor Bassi Ferreira, Soares Isadora Mello Vilarinho, Salles Alessandro Guimarães, Antunes Lívia Azeredo Alves, Segato Raquel Assed Bezerra, Silva Léa Assed Bezerra da, Küchler Erika Calvano, Antunes Leonardo Santos, Sousa-Neto Manoel Damião
Department of Restorative Dentistry, School of Dentistry of Ribeirão Preto, USP- Universidade de São Paulo, Ribeirão Preto, SP, Brazil.
Clinical Research Unit, UFF - Universidade Federal Fluminense, Niterói, RJ, Brazil.
Braz Dent J. 2018 Mar-Apr;29(2):179-183. doi: 10.1590/0103-6440201802260.
Persistent apical periodontitis (AP) is a situation involving an inflammatory and immune response caused mainly by anaerobic polymicrobial infection of the root canal system and the outcome and follow-up of the root canal treatment has been reported as intimately related to host response. The apical periodontitis repair might be associated with genetic polymorphisms. This study aimed to evaluate the association between HIF1A genetic polymorphisms (rs2301113 and rs2057482) with PAP in Brazilian patients. Subjects with at least 1 year of follow-up after root canal therapy (RCT) were recalled. Sixty-four subjects with signs/symptoms of PAP and 84 subjects with root canal-treated teeth exhibiting healthy perirradicular tissues (healed) were included. Genomic DNA was extracted from saliva and used for HIF1A genotyping by real-time PCR. Genotype and allele frequencies were compared by c2 or Fisher's exact tests and odds ratio was implemented, using Epi Info 3.5.2. All tests were performed with an established alpha of 0.05. There was no association between allele and genotype distribution for HIF1As polymorphisms and PAP (p>0.05). The genetic polymorphisms in HIF1A were not associated with persistent apical periodontitis.
慢性根尖周炎（AP）是一种主要由根管系统的厌氧微生物感染引起的炎症和免疫反应情况，并且根管治疗的结果及随访已被报道与宿主反应密切相关。根尖周炎的修复可能与基因多态性有关。本研究旨在评估巴西患者中缺氧诱导因子1α（HIF1A）基因多态性（rs2301113和rs2057482）与慢性根尖周炎（PAP）之间的关联。对根管治疗（RCT）后至少随访1年的受试者进行召回。纳入64例有PAP体征/症状的受试者和84例根管治疗后根尖周组织健康（已愈合）的受试者。从唾液中提取基因组DNA，并通过实时聚合酶链反应用于HIF1A基因分型。使用Epi Info 3.5.2通过卡方检验或Fisher精确检验比较基因型和等位基因频率，并计算优势比。所有检验均在设定的α为0.05的情况下进行。HIF1A多态性的等位基因和基因型分布与PAP之间无关联（p>0.05）。HIF1A基因多态性与慢性根尖周炎无关。
