Specific Formation Department, School of Dentistry of Nova Friburgo, Fluminense Federal University, Nova Friburgo, Brazil.
Clinical Research Unit, Fluminense Federal University, Niterói, Brazil.
Int Endod J. 2021 Jan;54(1):38-45. doi: 10.1111/iej.13401. Epub 2020 Sep 26.
To evaluate the association between the promoter region of defensin beta 1 (DEFB1) genetic polymorphisms and persistent apical periodontitis (PAP) in Brazilian patients.
Seventy-three patients with post-treatment PAP (PAP group) and 89 patients with root filled teeth with healed and healthy periradicular tissues (healed group) were included (all teeth had apical periodontitis lesions at the beginning of the treatment). Patients who had undergone at least 1 year of follow-up after root canal treatment were recalled, and their genomic DNA was extracted from saliva. Two single nucleotide polymorphisms (SNPs) in DEFB1 at the g. -52G>A (rs1799946) and g. -20G>A (rs11362) positions were analysed using real-time polymerase chain reaction. The chi-squared test was performed, and the odds ratios were calculated using Epi Info 3.5.2. Logistic regression analysis in the codominant model, using the time of follow-up as a variable, was used to evaluate the SNP-SNP interaction. All tests were performed with an established alpha of 0.05 (P = 0.05).
For the rs11362 polymorphism in the codominant and recessive models, patients who carried two copies of the T allele had a significantly lower risk of developing PAP (P = 0.040 and P = 0.031, respectively). For the rs1799946 polymorphism in DEFB1 in the codominant and recessive models, carrying one copy of the T allele significantly increased the risk of developing PAP (P = 0.007 and P = 0.031, respectively). In the logistic regression, both polymorphisms were associated with PAP as well as the SNP-SNP interaction (P < 0.0001).
Polymorphisms in DEFB1 genes were associated with the development of post-treatment persistent apical periodontitis.
评估防御素β 1（DEFB1）基因启动子区域遗传多态性与巴西患者持续性根尖周炎（PAP）之间的关联。
纳入 73 例治疗后 PAP 患者（PAP 组）和 89 例根充牙有愈合和健康牙周组织的患者（愈合组）（所有牙齿在治疗开始时均有根尖周炎病变）。对接受根管治疗后至少随访 1 年的患者进行召回，并从唾液中提取其基因组 DNA。使用实时聚合酶链反应分析 DEFB1 基因 g. -52G>A（rs1799946）和 g. -20G>A（rs11362）位置的两个单核苷酸多态性（SNP）。采用卡方检验，使用 Epi Info 3.5.2 计算优势比。使用共显性模型进行逻辑回归分析，以随访时间作为变量，评估 SNP-SNP 相互作用。所有检验的显著性水平均设为 0.05（P=0.05）。
对于 rs11362 多态性的共显性和隐性模型，携带两个 T 等位基因的患者 PAP 发生风险显著降低（P=0.040 和 P=0.031）。对于 DEFB1 基因 rs1799946 多态性的共显性和隐性模型，携带一个 T 等位基因显著增加了 PAP 的发病风险（P=0.007 和 P=0.031）。在逻辑回归中，两种多态性均与 PAP 以及 SNP-SNP 相互作用相关（P<0.0001）。
DEFB1 基因多态性与治疗后持续性根尖周炎的发生有关。
