Lehrach H, Frischauf A M, Philipson L
Prog Clin Biol Res. 1985;177:7-15.
The rapid progress in molecular cloning and DNA analysis techniques, together with the use of cloned DNA probes from specific chromosomes of the human genome might allow localisation and ultimately identification of genes defined by single mutations. The discrepancy between genetic dimensions expressed in centimorgans, each corresponding to millions of base pairs and distances easily accessible by molecular techniques amounting to at the most hundreds of kilobase pairs may be bridged with some new cloning techniques partially developed at the European Molecular Biology Laboratory. These techniques were designed to allow rapid cloning and analysis of large regions of mammalian genomes.
分子克隆和DNA分析技术的迅速发展，以及使用来自人类基因组特定染色体的克隆DNA探针，可能会实现由单一突变所定义基因的定位，并最终实现其鉴定。以厘摩表示的遗传距离（每1厘摩对应数百万碱基对）与分子技术能够轻易获取的最多只有几百千碱基对的距离之间存在差异，欧洲分子生物学实验室部分研发的一些新克隆技术或许能够弥合这一差异。这些技术旨在实现对哺乳动物基因组大片段的快速克隆和分析。
