CVAS, Jhang Campus, University of Veterinary and Animal Sciences, Lahore, Pakistan.
Department of Biochemistry, Government College University, Faisalabad, Pakistan.
Sci Total Environ. 2018 Jun 1;626:1295-1309. doi: 10.1016/j.scitotenv.2018.01.066. Epub 2018 Feb 19.
Vibrio fischeri bioluminescence inhibition bioassay (VFBIA) has been widely applied for the monitoring of toxicity on account of multiple advantages encompassing shorter test duration, sensitive, cost-effective and ease of operation. Moreover, this bioassay found to be equally applicable to all types of matrices (organic & inorganic compounds, metals, wastewater, river water, sewage sludge, landfill leachate, herbicides, treated wastewater etc.) for toxicity monitoring. This review highlights the apparent significance of Vibrio fischeri bioluminescence inhibition assay for ecotoxicological screening and evaluation of diverse chemical substances toxicity profile. The biochemical and genetic basis of the bioluminescence assay and its regulatory mechanism have been concisely discussed. The basic test protocol with ongoing improvements, widespread applications, typical advantages and probable limitations of the assay have been overviewed. The sensitivity of VFBIA and toxicity bioassays has also been compared.
发光杆菌生物发光抑制生物测定法（VFBIA）由于具有测试时间短、灵敏、经济高效和易于操作等多种优势，已被广泛应用于毒性监测。此外，该生物测定法被发现同样适用于所有类型的基质（有机和无机化合物、金属、废水、河水、污水污泥、垃圾渗滤液、除草剂、处理后的废水等）进行毒性监测。本综述强调了发光杆菌生物发光抑制测定法在生态毒理学筛选和评估各种化学物质毒性特征方面的明显重要性。简要讨论了生物发光测定法的生化和遗传基础及其调控机制。综述了基本测试方案及其不断改进、广泛应用、典型优势和可能的局限性。还比较了 VFBIA 和毒性生物测定的灵敏度。
