Chandra S, Morrison G H
Science. 1985 Jun 28;228(4707):1543-4. doi: 10.1126/science.2990033.
Both elemental distribution and ion transport in cultured cells have been imaged by ion microscopy. Morphological and chemical information was obtained with a spatial resolution of approximately 0.5 micron for sodium, potassium, calcium, and magnesium in freeze-fixed, cryofractured, and freeze-dried normal rat kidney cells and Chinese hamster ovary cells. Ion transport was successfully demonstrated by imaging Na+-K+ fluxes after the inhibition of Na+- and K+ -dependent adenosine triphosphatase with ouabain. This method allows measurements of elemental (isotopic) distribution to be related to cell morphology, thereby providing the means for studying ion distribution and ion transport under different physiological, pathological, and toxicological conditions in cell culture systems.
离子显微镜已对培养细胞中的元素分布和离子转运进行了成像。在冷冻固定、冷冻断裂和冷冻干燥的正常大鼠肾细胞及中国仓鼠卵巢细胞中，钠、钾、钙和镁的形态学和化学信息以约0.5微米的空间分辨率获得。在用哇巴因抑制钠钾依赖性三磷酸腺苷酶后，通过对钠钾通量成像成功证明了离子转运。该方法可将元素（同位素）分布的测量与细胞形态相关联，从而为研究细胞培养系统中不同生理、病理和毒理条件下的离子分布和离子转运提供手段。
