Marakhova I I, Pospelova T V, Vinogradova T A, Vereninov A A, Ignatova T N
Tsitologiia. 1985 Sep;27(9):1011-20.
Cation transport has been investigated in three lines of Chinese ovary cells CHO-K1 during the cell culture growth. With the increase in the cell density potassium and sodium contents decreased from 1.2 to 0.8-0.5 and from 0.5 to 0.15-0.1 mmole/g protein, respectively. The time courses of potassium and sodium changes were different, and the increase in intracellular K/Na ratio from 1.5-2.0 to 5-10 with the increase in cell density was revealed. The rubidium influx was found to decrease during the culture growth mainly due to the decrease in ouabain-inhibitable and (ouabain + furosemide)- non-inhibitable influxes. The changes in cation fluxes and cation contents were observed in transformed cells without contact inhibition of division and were considered as a manifestation of density-dependent alterations of plasma membrane.
在细胞培养生长过程中，对三株中国仓鼠卵巢细胞CHO-K1系中的阳离子转运进行了研究。随着细胞密度的增加，钾和钠的含量分别从1.2降至0.8 - 0.5以及从0.5降至0.15 - 0.1毫摩尔/克蛋白质。钾和钠变化的时间进程不同，并且随着细胞密度的增加，细胞内钾/钠比值从1.5 - 2.0增加到5 - 10。发现铷流入在培养生长过程中减少，主要是由于哇巴因抑制性和（哇巴因 + 呋塞米）非抑制性流入的减少。在没有分裂接触抑制的转化细胞中观察到阳离子通量和阳离子含量的变化，并被认为是质膜密度依赖性改变的一种表现。
