Gloor Gregory B, Wong Ruth Grace, Allen-Vercoe Emma, Dinculescu Vincent, Pignanelli Michael, Bogiatzi Chrysi, Reid Gregor, Spence J David
Dept. Biochemistry, Schulich School of Medicine and Dentistry, Western University, London, Canada.
Dept. Molecular and Cell Biology, University of Guelph, Guelph, Canada.
Data Brief. 2018 May 11;19:481-485. doi: 10.1016/j.dib.2018.05.032. eCollection 2018 Aug.
This work was conducted to characterize the 16S rRNA gene profile of a cohort of patients with traditional risk factors for developing atherosclerosis. The patients in the cohort were divided into two extremes; those predicted to develop extreme atherosclerosis who did not (Protected), and those predicted not to develop atherosclerosis who did (Unexplained). Bacterial DNA was isolated from stool and saliva and this was used to determine the V4 variable region of the 16S rRNA gene sequence composition of the samples in triplicate.
开展这项工作是为了描绘一组具有动脉粥样硬化传统危险因素患者的16S rRNA基因图谱。该队列中的患者被分为两个极端组；那些预计会发生严重动脉粥样硬化但未发生的患者（受保护组），以及那些预计不会发生动脉粥样硬化但却发生了的患者（不明原因组）。从粪便和唾液中分离细菌DNA，并将其用于一式三份地测定样本中16S rRNA基因序列组成的V4可变区。
