Konwalinka G, Peschel C, Schmalzl F, Michelmayer G, Peer L, Huber H, Braunsteiner H
Acta Med Austriaca. 1979;6(5):219-22.
After incubation of bone marrow cells obtained from normal subjects and patients with pancytopenia in the double layer agar culture (Robinson-Pike), the clusters of different size and the colonies were counted on days 2, 3, 5, 7 and 10. Aplastic anemia and two types of preleukemia could be distinguished by a different growth pattern. A high incidence of small clusters (4-12 cells) was found in patients with aplastic anemia on days 2 and 3, whereas these aggregates were markedly reduced in preleukemia of type B. In preleukemia of type A an excessive number of small clusters was obtained. The morphology of cells in clusters and colonies was studied by cytochemical staining and electronmicroscopy. In preleukemia marked defects of maturation were demonstrable in the cells.
将从正常受试者和全血细胞减少症患者获取的骨髓细胞在双层琼脂培养（罗宾逊-派克法）中孵育后，在第2、3、5、7和10天对不同大小的集落和克隆进行计数。再生障碍性贫血和两种类型的白血病前期可通过不同的生长模式加以区分。再生障碍性贫血患者在第2天和第3天出现小集落（4 - 12个细胞）的高发生率，而在B型白血病前期这些聚集体明显减少。在A型白血病前期获得了过多的小集落。通过细胞化学染色和电子显微镜研究了集落和克隆中细胞的形态。在白血病前期，细胞中可显示出明显的成熟缺陷。
