Department of Clinical Nutrition, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai 200233, P.R. China.
Department of Emergency, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai 200233, P.R. China.
Oncol Rep. 2018 Aug;40(2):999-1007. doi: 10.3892/or.2018.6497. Epub 2018 Jun 14.
γ-tocotrienol (γ-T3), a tocotrienol isoform belonging to the vitamin E family, has been revealed to exert inhibitory effects on proliferation, migration and invasion in human gastric cancer cells. However, its precise mechanism of action is still unclear and needs to be further tested. Cyclooxygenase-2 (COX-2) is well known for its key role in promoting the migration and invasion abilities of human gastric cancer cells. In light of these data, our study aimed to validate whether the inhibitory actions of γ-T3 could be achieved by downregulation of COX-2 activity in vitro. In the present study, a Cell Counting Kit-8 (CCK-8) assay was performed to observe proliferation in human gastric cancer cells (SGC-7901 and MGC-803 cells), and wound healing and Transwell chamber assays were performed to detect migration and invasion. Western blot analyses were performed to analyse the relative expression of COX-2, matrix metalloproteinases-2 and -9 (MMP-2 and MMP-9) proteins, and enzyme-linked immunosorbent assays (ELISA) were used to determine the exocrine roles of MMP-2 and MMP-9. The results revealed that γ-T3 exerted significant inhibitory effects on proliferation, migration, invasion and COX-2 protein expression, as well as on exocrine functions of MMP-2 and MMP-9 in SGC-7901 and MGC-803 cells. Therefore, our results indicated that γ-T3 exerts inhibitory effects on migration and invasion, which may be mediated through downregulation of COX-2 expression in SGC-7901 and MGC-803 cells.
γ-生育三烯酚（γ-T3）是维生素 E 家族的一种生育三烯酚异构体，已被证实对人胃癌细胞的增殖、迁移和侵袭具有抑制作用。然而，其确切的作用机制仍不清楚，需要进一步验证。环氧化酶-2（COX-2）在促进人胃癌细胞迁移和侵袭能力方面的作用是众所周知的。有鉴于此，我们的研究旨在验证 γ-T3 的抑制作用是否可以通过下调 COX-2 的活性来实现。在本研究中，通过细胞计数试剂盒-8（CCK-8）测定观察人胃癌细胞（SGC-7901 和 MGC-803 细胞）的增殖，通过划痕愈合和 Transwell 室测定检测迁移和侵袭。通过 Western blot 分析检测 COX-2、基质金属蛋白酶-2 和 -9（MMP-2 和 MMP-9）蛋白的相对表达，通过酶联免疫吸附测定（ELISA）测定 MMP-2 和 MMP-9 的外分泌作用。结果表明，γ-T3 对 SGC-7901 和 MGC-803 细胞的增殖、迁移、侵袭以及 COX-2 蛋白表达和 MMP-2 和 MMP-9 的外分泌功能均有显著抑制作用。因此，我们的结果表明，γ-T3 对迁移和侵袭具有抑制作用，这可能是通过下调 SGC-7901 和 MGC-803 细胞中 COX-2 的表达来介导的。
