Dueland S, Bouillon R, Van Baelen H, Pedersen J I, Helgerud P, Drevon C A
Am J Physiol. 1985 Jul;249(1 Pt 1):E1-5. doi: 10.1152/ajpendo.1985.249.1.E1.
A protein with high affinity for vitamin D3 and 25-hydroxyvitamin D3 in rat mesenteric lymph has been studied. Mesenteric lymph was collected after duodenal instillation of radiolabeled vitamin D3 and 25-hydroxyvitamin D3. As previously described, approximately 10% of vitamin D3 (S. Dueland, J.I. Pedersen, P. Helgerud, and C.A. Drevon, J. Biol. Chem. 257: 146-150, 1982) and 95% of 25-hydroxyvitamin D3 (S. Dueland, J.I. Pedersen, P. Helgerud, and C.A. Drevon, Am. J. Physiol. 245 (Endocrinol. Metab. 8): E463-E467, 1983) recovered in mesenteric lymph were associated with the alpha-globulin fractions. The radioactive vitamin D3 recovered in the lymph fraction with d greater than 1.006 (free of chylomicrons) coeluted with purified rat serum binding protein for vitamin D and its metabolites (DBP) from an antirat DBP column. The results obtained by immunoblotting after sodium dodecyl sulfate polyacrylamide gel electrophoresis showed that this protein in mesenteric lymph had molecular weight and immunological properties identical with purified serum DBP. Purified serum DBP labeled with 125I was injected intravenously and mesenteric lymph was collected. in lymph, suggesting that DBP may be transferred from blood to mesenteric lymph and that plasma and lymph DBP may have a similar origin.
对大鼠肠系膜淋巴中与维生素D3和25-羟基维生素D3具有高亲和力的一种蛋白质进行了研究。在十二指肠灌注放射性标记的维生素D3和25-羟基维生素D3后收集肠系膜淋巴。如先前所述，肠系膜淋巴中回收的约10%的维生素D3（S. 杜兰德、J.I. 佩德森、P. 赫尔格鲁德和C.A. 德雷冯，《生物化学杂志》257: 146 - 150, 1982）和95%的25-羟基维生素D3（S. 杜兰德、J.I. 佩德森、P. 赫尔格鲁德和C.A. 德雷冯，《美国生理学杂志》245(内分泌与代谢8): E463 - E467, 1983）与α-球蛋白组分相关。在密度大于1.006的淋巴组分（不含乳糜微粒）中回收的放射性维生素D3与来自抗大鼠DBP柱的纯化大鼠血清维生素D及其代谢产物结合蛋白（DBP）共洗脱。十二烷基硫酸钠聚丙烯酰胺凝胶电泳后免疫印迹得到的结果表明，肠系膜淋巴中的这种蛋白质具有与纯化血清DBP相同的分子量和免疫学特性。静脉注射用125I标记的纯化血清DBP并收集肠系膜淋巴。在淋巴中，提示DBP可能从血液转移至肠系膜淋巴，且血浆和淋巴DBP可能有相似的来源。
