Lim D J, Flock A
Am J Otolaryngol. 1985 May-Jun;6(3):153-62. doi: 10.1016/s0196-0709(85)80078-8.
Using the enzyme dissociation technique, the authors successfully isolated strial marginal cells and intermediate cells from guinea pig and chinchilla cochleas. Dissociated cells were maintained for in vitro observation under the light microscope for up to 2 hours without gross evidence of cell damage. Such single cells were successfully prepared for scanning or transmission electron microscopic observation, which showed excellent cell shape and preservation of cell organelles. However, there was evidence of increased vesiculation in the dissociated marginal cells, which is interpreted to be evidence of increased pinocytotic activity.
作者采用酶解离技术，成功地从豚鼠和毛丝鼠的耳蜗中分离出血管纹边缘细胞和中间细胞。解离后的细胞在光学显微镜下进行体外观察，长达2小时，未见明显的细胞损伤迹象。这些单细胞成功地制备用于扫描或透射电子显微镜观察，显示出良好的细胞形态和细胞器保存。然而，有证据表明解离后的边缘细胞中囊泡形成增加，这被解释为胞饮活性增加的证据。
