Watanabe K, Ogawa A
Ann Otol Rhinol Laryngol. 1984 May-Jun;93(3 Pt 1):262-6. doi: 10.1177/000348948409300315.
Carbonic anhydrase activity was demonstrated ultracytochemically in the dark cells of the vestibular labyrinth and in the stria vascularis of the guinea pig. Reaction products were found both in the dark cells and in the stria vascularis. Localization of carbonic anhydrase was very similar between the dark cells and the intermediate cells, but the marginal cells had a different localization pattern. In both the dark cells and the intermediate cells, the reaction products were deposited in the cytoplasm, especially in the infoldings. In the marginal cells, carbonic anhydrase activity was observed on the lateral plasma membrane of the apical area and in a few apical vesicles, but was not detected in the infoldings of the deep portion. Reaction products were also observed in the intercellular spaces between the marginal cells. No reaction products were observed in the basal cells and transition cells. Carbonic anhydrase activity was observed in the cytoplasm of capillary endothelial cells and the fibrocytes of the spiral ligament. It is considered that carbonic anhydrase may play a major role in water and electrolyte transport in both the dark cells and stria vascularis.
采用超微细胞化学方法在豚鼠前庭迷路的暗细胞和血管纹中证实了碳酸酐酶活性。在暗细胞和血管纹中均发现了反应产物。暗细胞和中间细胞中碳酸酐酶的定位非常相似，但边缘细胞具有不同的定位模式。在暗细胞和中间细胞中，反应产物都沉积在细胞质中，尤其是在褶皱处。在边缘细胞中，在顶端区域的外侧质膜和一些顶端小泡中观察到碳酸酐酶活性，但在深部的褶皱中未检测到。在边缘细胞之间的细胞间隙中也观察到了反应产物。在基底细胞和过渡细胞中未观察到反应产物。在毛细血管内皮细胞的细胞质和螺旋韧带的纤维细胞中观察到了碳酸酐酶活性。据认为，碳酸酐酶可能在暗细胞和血管纹中的水和电解质转运中起主要作用。
