[硒酵母对细颗粒物诱导的大鼠肺损伤的干预作用]

[Intervention effects of selenium yeast on fine particulate matters-induced lung injury in rats].

作者信息

Liu Jie, Zeng Xuejiao, Yang Yingying, Jiang Rongfang, Zhao Jinzhuo, Song Weimin

机构信息

Department of Environmental Health, School of Public Health, Fudan University, Shanghai 200032, China.

出版信息

Wei Sheng Yan Jiu. 2017 Mar;46(2):219-231.

PMID:29903097

Abstract

OBJECTIVE

To evaluate if selenium yeast could inhibit the rat lung injury induced by ambient fine particulate matter( PM_(2. 5) ).

METHODS

Fifty-six male SpragueDawley rats were randomly allocated in seven groups( n = 8 each). Saline control group, the rats were exposed to 0. 9% saline by instillation. PM_(2. 5) exposure group, rats were exposed to PM_(2. 5) by intra-tracheal instillation every other day for three times with the accumulated dose of 40 mg/kg. Selenium yeast treatment groups, three groups of rat were exposed to PM_(2. 5) . Then the rats were given low, middle and high dose of selenium yeast, and the doses were 8. 75, 17. 5 and 35 mg/kg, respectively. High dose selenium yeast control group, rats were given high dose of selenium yeast only. Solvent control group, therats were given 1% carboxymethyl cellulose. Saline and PM_(2. 5) were given in the first week. In the second week, selenium yeast and solvent were given by gavage. The rats were sacrificed 24 hours after the last gavage. The bronchoalveolar lavage fluid( BALF)was collected to count the neutrophils numbers and analyze the markers related to inflammation, oxidative stress and cell damage. The lung lobe that was not been lavaged was processed for light microscopic examination.

RESULTS

The proportions of neutrophils in BALF and the pathologic scores of lung in PM_(2. 5) - exposed groups were significantly higher than control( P < 0. 05). Selenium yeast treatment caused decrease in tumor necrosis factor-α( TNF-α), interleukin-1β( IL-1β), lactate dehydrogenase( LDH), total protein( TP), alkaline phosphatase( AKP) and malondialdehyde( MDA) compared with the only PM_(2. 5) exposure group. Meanwhile, the dose-dependent increase in totalsuperoxide dismutase( T-SOD) and glutathione peroxidase( GSH-Px) activities were observed. There were no significant differences among the groups of saline control group, high dose selenium yeast control group and solvent control group.

CONCLUSION

Selenium yeast treatment may protect against acute injury induced by PM_(2. 5) in rat lung.

摘要

目的

评估硒酵母是否能抑制环境细颗粒物（PM₂.₅）诱导的大鼠肺损伤。

方法

56只雄性Sprague-Dawley大鼠随机分为7组（每组n = 8）。生理盐水对照组，大鼠经滴注给予0.9%生理盐水。PM₂.₅暴露组，大鼠每隔一天经气管内滴注PM₂.₅，共3次，累积剂量为40 mg/kg。硒酵母治疗组，3组大鼠暴露于PM₂.₅。然后分别给予低、中、高剂量的硒酵母，剂量分别为8.75、17.5和35 mg/kg。高剂量硒酵母对照组，大鼠仅给予高剂量硒酵母。溶剂对照组，大鼠给予1%羧甲基纤维素。第一周给予生理盐水和PM₂.₅。第二周，通过灌胃给予硒酵母和溶剂。在最后一次灌胃饲胃管给药24小时后处死大鼠。收集支气管肺泡灌洗液（BALF）以计数中性粒细胞数量，并分析与炎症、氧化应激和细胞损伤相关的标志物。对未进行灌洗的肺叶进行光镜检查。

结果

PM₂.₅暴露组BALF中中性粒细胞比例和肺病理评分显著高于对照组（P < 0.05）。与仅PM₂.₅暴露组相比，硒酵母治疗使肿瘤坏死因子-α（TNF-α）、白细胞介素-1β（IL-1β）、乳酸脱氢酶（LDH）、总蛋白（TP）、碱性磷酸酶（AKP）和丙二醛（MDA）降低。同时，观察到总超氧化物歧化酶（T-SOD）和谷胱甘肽过氧化物酶（GSH-Px）活性呈剂量依赖性增加。生理盐水对照组、高剂量硒酵母对照组和溶剂对照组之间无显著差异。