Zhang Nan, Fan Sai, Xue Ying, Liu Ping, Liu Wei, Wu Guohua, Zhao Rong
Beijing Center for Disease Control and Prevention, Beijing 100013, China.
Wei Sheng Yan Jiu. 2017 Jan;46(1):89-93.
To develop a method for determination of 8 veterinary drug residues in chicken matrix by Qu ECh ERS-ultra performance liquid chromatography tandem mass spectrometry.
Chicken samples were extracted with acid acetonitrile, salting out, and then the organic phase were cleaned up by C18 and PSA. A Waters ACQUITYTM BEH C18column( 100 mm × 2. 1 mm × 1. 7 μm) was used for LC separation, ESI positive ion scan was used with multiple reaction monitoring( MRM)mode and quantified by matrix-matched external standard method.
At the spiked level of 1, 2 and 4 μg / kg, the recoveries of each compound were in the range of 81. 2%- 94. 1% with the relative standard deviations of 4. 2%- 14. 3%. The qualitative limits of detections were 0. 1- 0. 2 μg / kg and the quantitative limits were 0. 2- 0. 5 μg / kg for the 8 targets compounds. The established method was applied to the detection of the 8targets compounds in 30 chicken samples, and none of the 8 veterinary drugs exceeded the residue limits.
The method is simple, rapid, high sensitivity and good stability, with a wide variety and a certain development. It can be used for the daily monitoring of the veterinary drug residues in chicken.
建立一种采用Qu ECh ERS-超高效液相色谱串联质谱法测定鸡肉基质中8种兽药残留的方法。
鸡肉样品用酸性乙腈提取、盐析，然后有机相经C18和PSA净化。采用Waters ACQUITYTM BEH C18柱（100 mm×2.1 mm×1.7μm）进行液相色谱分离，采用电喷雾电离正离子扫描，多反应监测（MRM）模式，基质匹配外标法定量。
在1、2和4μg/kg的加标水平下，各化合物的回收率在81.2% - 94.1%之间，相对标准偏差为4.2% - 14.3%。8种目标化合物的定性检出限为0.1 - 0.2μg/kg，定量限为0.2 - 0.5μg/kg。将所建立的方法应用于30份鸡肉样品中8种目标化合物的检测，8种兽药均未超过残留限量。
该方法简便、快速、灵敏度高、稳定性好，具有较宽的适用范围和一定的拓展性。可用于鸡肉中兽药残留的日常监测。
