Fan Sai, Zhang Nan, Liu Ping, Li Liping, Wu Guohua, Zhao Rong
Beijing Center for Disease Prevention and Control, Beijing Research Center for Preventive Medicine, Key Laboratory of Diagnostic and Traceability Technologies for Food Poisoning, Beijing 100013, China.
Wei Sheng Yan Jiu. 2020 Sep;49(5):829-867. doi: 10.19813/j.cnki.weishengyanjiu.2020.05.023.
To establish an analytical method for determination of 20 kinds of β-receptor blockers residues in animal foods by ultra high performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS).
The samples of animal foods were enzymatic hydrolysis by trichloroacetic acid(TCA), purified by MCX column. The separation was performed on a Waters ACQUITYTM BEH C_(18 )column(100 mm×2. 1 mm, 1. 7 μm), then the target compounds were detected by UPLC-MS/MS with ESI positive ion scan in mode of multiple reaction monitoring(MRM) and quantified by matrix matched external standard method.
At the spiked level of 1, 2 and 4 μg/kg, the recoveries of each compound were in the range of 61. 9%-119. 1% with the relative standard deviations of 1. 5%-28. 4%(n=6). The qualitative limits of detections were 0. 01-0. 15 μg/kg and the quantitative limits were 0. 03-0. 50 μg/kg for the 20 targets compounds. By using the established method, the target compounds in 30 animal foods were detected, and no excessive veterinary drug residue were detected.
The established method is simple, rapid, high sensitivity and good stability, with a wide variety and a certain development. It can provide more convenient and fast detection method support for the daily monitoring of veterinary drug residues in animal foods.
