[SIRT1和APOC3单核苷酸多态性与非酒精性脂肪性肝病的关联]

[Association of single nucleotide polymorphism of SIRT1 and APOC3 with nonalcoholic fatty liver disease].

作者信息

Song Xiaochao, Song Chunli, Fan Li, Ma Qinghua, Mao Jianliang, Xu Wenxin, Li Xinli

机构信息

Department of Nutrition and Food Hygiene, School of Public Health, Soochow University, Suzhou 215123, China.

出版信息

Wei Sheng Yan Jiu. 2017 Jul;46(4):527-532.

PMID:29903170

Abstract

OBJECTIVE

To explore the association of silent information regulator 1( SIRT1) rs12778366 and apolipoprotein C3( APOC3) rs2854116 gene polymorphisms with the susceptibility of nonalcoholic fatty liver disease( NAFLD).

METHODS

One hundred and thirty two NAFLD patients and 252 healthy controls were enrolled in our present study according to the ultrasound diagnosis and physical examination. Venousblood samples were obtained in the morning after an overnight fast, and the samples were used to analyze the biochemical index, relating to hepatic enzymes, blood lipid and blood glucose metabolism. DNA was extracted from whole blood, and polymerase chain reaction( PCR) and mass ARRAY were used to determine the genotypes of target genes.

RESULTS

Compared with TT genotype carriers, the SIRT1 genotype rs12778366 increased the risk of NAFLD, with OR = 1. 126( 95% CI 0. 673-1. 886)( P > 0. 05). The APOC3rs2854116 TC + TT genotype increased the risk of NAFLD compared with CC genotype( OR = 1. 044, 95% CI 0. 601-1. 814, P > 0. 05). The adjusted odds ratios had no significant changes after adjusting for gender, age and BMI. Logistic regression showed that TG, body mass index( BMI), FPG, WC and UA were the independent risk factors for NAFLD( P < 0. 05), but the polymorphisms of SIRT1 rs12778366 and APOC3 rs2854116 had no significant relationship with the risk of NAFLD.

CONCLUSION

SIRT1 rs12778366 and APOC3 rs2854116 polymorphisms were not associated with NAFLD susceptibility.

摘要

目的

探讨沉默信息调节因子1（SIRT1）rs12778366和载脂蛋白C3（APOC3）rs2854116基因多态性与非酒精性脂肪性肝病（NAFLD）易感性的关系。

方法

根据超声诊断和体格检查，选取132例NAFLD患者和252例健康对照纳入本研究。空腹过夜后于清晨采集静脉血样本，用于分析与肝酶、血脂和血糖代谢相关的生化指标。从全血中提取DNA，采用聚合酶链反应（PCR）和质谱分析确定靶基因的基因型。

结果

与TT基因型携带者相比，SIRT1基因rs12778366基因型增加了NAFLD的发病风险，OR = 1.126（95%CI 0.673 - 1.886）（P > 0.05）。与CC基因型相比，APOC3 rs2854116的TC + TT基因型增加了NAFLD的发病风险（OR = 1.044，95%CI 0.601 - 1.814，P > 0.05）。在校正性别、年龄和BMI后，调整后的比值比无显著变化。Logistic回归显示，甘油三酯（TG）、体重指数（BMI）、空腹血糖（FPG）、腰围（WC）和尿酸（UA）是NAFLD的独立危险因素（P < 0.05），但SIRT1 rs12778366和APOC3 rs2854116的多态性与NAFLD的发病风险无显著关系。