Li Min-Rui, Zhang Sheng-Hong, Chao Kang, Liao Xian-Hua, Yao Jia-Yan, Chen Min-Hu, Zhong Bi-Hui
Min-Rui Li, Sheng-Hong Zhang, Kang Chao, Xian-Hua Liao, Jia-Yan Yao, Min-Hu Chen, Bi-Hui Zhong, Division of Gastroenterology, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, Guangdong Province, China.
World J Gastroenterol. 2014 Oct 14;20(38):14010-7. doi: 10.3748/wjg.v20.i38.14010.
To investigate the relationship between Apolipoprotein C3 (APOC3) (-455T>C) polymorphism and nonalcoholic fatty liver disease (NAFLD) in the Southern Chinese Han population.
In this prospective case-control study, we recruited 300 NAFLD patients and 300 healthy controls to a cohort representing Southern Chinese Han population at The First Affiliated Hospital, Sun Yat-sen University, from January to December 2012. Polymerase chain reaction-restriction fragment length polymorphism and DNA sequencing were used to genotype the APOC3 (-455T>C) variants.
After adjusting for age, gender, and body-mass index, TC and CC genotypes were found to increase the susceptibility to NAFLD compared to the TT genotype, with adjusted odds ratios (ORs) of 1.77 (95%CI: 1.16-2.72) and 2.80 (95%CI: 1.64-4.79), respectively. Further stratification analysis indicated that carriers of the CC genotype was more susceptible to insulin resistance (IR) than those of the TT genotype, with an OR of 3.24 (95%CI: 1.52-6.92). The CC genotype also was associated with a significantly higher risk of hypertension, hypertriglyceridemia, and low levels of high-density lipoprotein cholesterol (HDL) (P < 0.05). No association was found between the APOC3 (-455T>C) polymorphism and obesity, impaired glucose tolerance, hyperuricemia, hypercholesterolemia, or high levels of low-density lipoprotein cholesterol (LDL) (P > 0.05).
APOC3 (-455T>C) genetic variation is involved in the susceptibility to developing NAFLD, IR, hypertension, hypertriglyceridemia, and low HDL in the Southern Chinese Han population.
探讨载脂蛋白C3（APOC3）（-455T>C）基因多态性与中国南方汉族人群非酒精性脂肪性肝病（NAFLD）的关系。
在这项前瞻性病例对照研究中，2012年1月至12月，我们在中山大学附属第一医院招募了300例NAFLD患者和300例健康对照，组成一个代表中国南方汉族人群的队列。采用聚合酶链反应-限制性片段长度多态性和DNA测序技术对APOC3（-455T>C）变异进行基因分型。
在调整年龄、性别和体重指数后，发现TC和CC基因型与TT基因型相比增加了患NAFLD的易感性，调整后的比值比（OR）分别为1.77（95%CI：1.16-2.72）和2.80（95%CI：1.64-4.79）。进一步的分层分析表明，CC基因型携带者比TT基因型携带者更易患胰岛素抵抗（IR），OR为3.24（95%CI：1.52-6.92）。CC基因型还与高血压、高甘油三酯血症和高密度脂蛋白胆固醇（HDL）水平低的风险显著相关（P<0.05）。未发现APOC3（-455T>C）基因多态性与肥胖、糖耐量受损、高尿酸血症、高胆固醇血症或低密度脂蛋白胆固醇（LDL）水平高之间存在关联（P>0.05）。
APOC3（-455T>C）基因变异与中国南方汉族人群患NAFLD、IR、高血压、高甘油三酯血症和低HDL的易感性有关。
