Song Chunli, Yang Huanhuan, Song Xiaochao, Li Deming, Li Xinli
Department of Nutrition and Food Hygiene, School of Public Health, Medical College of Soochow University, Suzhou 215123, China.
Wei Sheng Yan Jiu. 2017 Nov;46(6):960-964.
To investigate the effects of apple polyphenol on cell proliferation and migration of breast cancer cell line MDA-MB-231, and explore the potential mechanism.
The breast cancer cells in the logarithmic phase were treated with 0, 50, 100, 200, 400 and 800 μg/m L of apple polyphenol for 24, 48 and 72 h, then Trypan blue staining was employed to detect cell vitality; CCK8 kit was used to determine cell growth and proliferation; cell migration ability was observed by scratch experiment, and the change of proteins expression level was assessed by Western blot.
Apple polyphenol could content-dependently inhibit breast cancer cell's vitality and proliferation between 0 and 800 μg/m L, decrease the proteins level of ubiquitinlike with PHD and ring finger domain 1( UHRF1) and matrix metalloproteinases-2( MMP2), and the protein levels of DNA methyltransferases 3 a( DNMT3 a) and DNMT3 b, themolecular targets of UHRF1, also decreased. Apple polyphenol inhibition of cell 's migration was more significant between 400 and 800 μg/mL.
Apple polyphenol inhibited the vitality, cell growth and proliferation, and migration of breast cancer cells MDA-MB-231 by decreasing the proteins expression of UHRF1 and MMP2, and the downstream targets of UHRF1, DNMT3 a and DNMT3 b, also decreased.
研究苹果多酚对乳腺癌细胞系MDA-MB-231细胞增殖和迁移的影响，并探讨其潜在机制。
将对数期乳腺癌细胞分别用0、50、100、200、400和800μg/mL的苹果多酚处理24、48和72小时，然后采用台盼蓝染色检测细胞活力；使用CCK8试剂盒测定细胞生长和增殖情况；通过划痕实验观察细胞迁移能力，并采用蛋白质免疫印迹法评估蛋白表达水平的变化。
在0至800μg/mL范围内，苹果多酚可呈浓度依赖性抑制乳腺癌细胞的活力和增殖，降低含PHD和指环结构域的泛素样蛋白1（UHRF1）和基质金属蛋白酶-2（MMP2）的蛋白水平，UHRF1的分子靶点DNA甲基转移酶3a（DNMT3a）和DNA甲基转移酶3b（DNMT3b）的蛋白水平也降低。在400至800μg/mL之间，苹果多酚对细胞迁移的抑制作用更显著。
苹果多酚通过降低UHRF1和MMP2的蛋白表达，以及UHRF1的下游靶点DNMT3a和DNMT3b的表达，抑制乳腺癌细胞MDA-MB-231的活力、细胞生长和增殖以及迁移。
