Zhang Yong, Yang Xueyan, Zhang Xinsheng, Xu Qing, Yu Xiaoming, Xue Changyong, Liu Yinghua
Department of Nutrition, Chinese PLA General Hospital, Beijing 100853, China.
Wei Sheng Yan Jiu. 2018 Jan;47(1):123-127.
To explore the effects of medium-chain fatty acid( MCFA) on high-density-lipoprotein( HDL) in serum, liver and small intestine in Sprague Dawley rats fed with high fat diet.
Thirty obese rats were divided into 3 groups randomly, and were fed high fat diet mixed with 2% octanoic acid, 2% decanoic acid, 2% oleic acid respectively for 8 weeks. The levels of blood HDL, apolipoprotein A1( ApoA1), apolipoprotein A2( ApoA2), triglyceride( TG), total cholesterol( TC), high-densitylipoprotein cholesterol( HDL-c), low-density lipoprotein cholesterol( HDL-c) in serum were measured at the fourth and eighth week. The levels of HDL, ApoA1, and ApoA2 in liver and small intestine were measured by ELISA. Real-time PCR was used to detect the mRNA expression of ApoA1 and ApoA2 in liver and small intestine from rats at the eighth week.
At the eighth week, significant decreases in levels of serum TG and TC were observed in octanoic acid and decanoic acid groups as compared with oleic acid group( P < 0. 05). Greater increases in levels of serum HDL and HDL-c/LDL-c were observed in octanoic acid group than in oleic acid group( P < 0. 05). Greater increases in protein expression of HDL and ApoA1 and in mRNA expression of ApoA1 in small intestine were observed in octanoic acid group than in oleic acid group( P < 0. 05).
MCFA can elevate the levels of HDL, HDL-c/LDL-c in serum, and increase the expressions of HDL and ApoA1 in small intestine.
探讨中链脂肪酸（MCFA）对高脂饮食喂养的斯普拉格-道利大鼠血清、肝脏和小肠中高密度脂蛋白（HDL）的影响。
将30只肥胖大鼠随机分为3组，分别用含2%辛酸、2%癸酸、2%油酸的高脂饲料喂养8周。在第4周和第8周时测定血清中HDL、载脂蛋白A1（ApoA1）、载脂蛋白A2（ApoA2）、甘油三酯（TG）、总胆固醇（TC）、高密度脂蛋白胆固醇（HDL-c）、低密度脂蛋白胆固醇（HDL-c）的水平。采用酶联免疫吸附测定法（ELISA）检测肝脏和小肠中HDL、ApoA1和ApoA2的水平。运用实时荧光定量聚合酶链反应（Real-time PCR）检测第8周大鼠肝脏和小肠中ApoA1和ApoA2的mRNA表达。
在第8周时，与油酸组相比，辛酸组和癸酸组血清TG和TC水平显著降低（P<0.05）。与油酸组相比，辛酸组血清HDL水平及HDL-c/LDL-c比值升高更明显（P<0.05）。与油酸组相比，辛酸组小肠中HDL和ApoA1蛋白表达及ApoA1 mRNA表达升高更明显（P<0.05）。
MCFA可提高血清中HDL、HDL-c/LDL-c水平，并增加小肠中HDL和ApoA1的表达。
